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LC–APCI–MS Quantitative Analysis of Curcumin in Rabbit Plasma

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Abstract

A simple and specific liquid chromatography–atmospheric pressure chemical ionization–mass spectrometry (LC–APCI–MS) method was developed and validated for quantitative analysis of curcumin in rabbit plasma. Chromatographic separation was carried out on Zorbax SB-C18 column, acetonitrile-methanol–water used as mobile phase with gradient elution. APCI source was applied and operated in negative ion mode, and selective ion monitoring mode used to quantify curcumin. The method showed excellent intra-assay and inter-assay precision (relative standard deviations and bias <15%) for quality control (QC) samples. This method was successfully applied to a pharmacokinetic study of curcumin after intravenous administration to rabbits.

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References

  1. Han S, Yang Y (2005) Dye Pigment 64:157–161

    Article  CAS  Google Scholar 

  2. Sharma OP (1976) Biochem Pharmacol 25:1811–1812

    Article  CAS  Google Scholar 

  3. Li J, Jiang Y, Wen J, Fan G, Wu Y, Zhang C (2009) Biomed Chromatogr 23:1201–1207

    Article  CAS  Google Scholar 

  4. Ma Z, Shayeganpour A, Brocks DR, Lavasanifar A, Samuel J (2007) Biomed Chromatogr 21:546–552

    Article  CAS  Google Scholar 

  5. Heath DD, Pruitt MA, Brenner DE, Rock CL (2003) J Chromatogr B Analyt Technol Biomed Life Sci 783:287–295

    Article  CAS  Google Scholar 

  6. Pak Y, Patek R, Mayersohn M (2003) J Chromatogr B Analyt Technol Biomed Life Sci 796:339–346

    Article  CAS  Google Scholar 

  7. Yang KY, Lin LC, Tseng TY, Wang SC, Tsai TH (2007) J Chromatogr B Analyt Technol Biomed Life Sci 853:183–189

    Article  CAS  Google Scholar 

  8. Liu A, Lou H, Zhao L, Fan P (2006) J Pharm Biomed Anal 40:720–727

    Article  CAS  Google Scholar 

  9. May LA, Tourkina E, Hoffman SR, Dix TA (2005) Anal Biochem 337:62–69

    Article  CAS  Google Scholar 

  10. Yu P, Gu Z, Guo X, Ran L, Cheng H, Zhang Cheng Z (2010) Chromatographia 72:151–155

    Article  CAS  Google Scholar 

  11. Huang S, Yu L, Shen P (2010) Chromatographia 72:63–69

    Article  CAS  Google Scholar 

  12. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Veterinary and Research (2001) Guidance for industry: bioanalytical method validation

Download references

Acknowledgment

This project was support by Wenzhou Municipal Science and Technology Agency-funded projects of Zhejiang Province (Y20090113).

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Authors and Affiliations

  1. The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China

    Xuezhi Yang, Guanyang Lin & Yuancai Zheng

  2. School of Basic Medical Sciences of Wenzhou Medical College, Wenzhou, 325035, China

    Jianshe Ma & Xu Li

  3. Analytical and Testing Center of Wenzhou Medical College, Wenzhou, 325035, China

    Yun Ye, Yuan Zhang & Xianqin Wang

Authors
  1. Xuezhi Yang
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  2. Jianshe Ma
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  3. Yun Ye
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  4. Yuan Zhang
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  5. Guanyang Lin
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  6. Yuancai Zheng
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  7. Xu Li
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  8. Xianqin Wang
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Corresponding author

Correspondence to Xianqin Wang.

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X. Yang and J. Ma contributed equally to this work.

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Yang, X., Ma, J., Ye, Y. et al. LC–APCI–MS Quantitative Analysis of Curcumin in Rabbit Plasma. Chromatographia 73, 605–608 (2011). https://doi.org/10.1007/s10337-011-1918-5

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  • DOI: https://doi.org/10.1007/s10337-011-1918-5

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