Abstract
Liensinine (LIE), isoliensinine (ISO) and neferine (NEF) from embryo of the seed of Nelumbo nucifera Gaertn were first separated and quantitatively analyzed by micellar electrokinetic chromatography with internal standard (IS) using a cationic surfactant, tetradecyltrimethyl ammonium bromide (TTAB). Complete separation of the analytes was optimally achieved within 15 min using 40 mM sodium dihydrogenphosphate (pH 6.40) containing 10 mM TTAB and 15% (v/v) methanol as buffer. Tetrahydropalmatine was used as IS to improve the repeatability and linearity relativity. The IS method resulted in excellent linearity, with correlation coefficients of regression equations of 0.9997, 0.9997 and 0.9992 for LIE, ISO and NEF, respectively. Finally, two sample extraction methods were used to investigate the contents of different parts of the embryos.
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This work was supported by the National Natural Science Foundation of China (grant nos. 20875040, J0730425).
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Zhu, J., Zhang, H., Qi, S. et al. Micellar Electrokinetic Chromatography Using a Cationic Surfactant for Rapid Separation and Determination of Bisbenzylisoquinoline Alkaloids from Embryo of the Seed of Nelumbo nucifera Gaertn. Chromatographia 73, 535–540 (2011). https://doi.org/10.1007/s10337-011-1911-z
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DOI: https://doi.org/10.1007/s10337-011-1911-z