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LC Assay for a HIV Tablet Containing Emtricitabine, Tenofovir Disoproxil Fumarate and Rilpivirine

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Abstract

A liquid chromatographic method with UV detection was developed for the assay of a tablet for HIV (human immunodeficiency virus) treatment containing three active components, which are emtricitabine, tenofovir disoproxil fumarate and rilpivirine. A Hypersil BDS-C18 column was used as stationary phase and the assay was performed with gradient elution using mobile phases containing acetonitrile, 0.2 M potassium dihydrogen phosphate and water. Dimethyl sulfoxide–distilled water (1:1) was used as solvent for the active components. The method proved to be selective, linear, repeatable, sensitive and easy to perform.

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Authors and Affiliations

  1. Laboratorium voor Farmaceutische Analyse, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, O&N2, Herestraat 49, PB 923, 3000, Louvain, Belgium

    Murali Pendela, Getu Weldu Kahsay, Jos Hoogmartens & Erwin Adams

  2. Laboratorium voor Farmacotechnologie en Biofarmacie, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, O&N2, Herestraat 49, PB 921, 3000, Louvain, Belgium

    Guy Van den Mooter

  3. Tibotec, Intercity Business Park, Mechelen Noord, Zone L, Gen. De Wittelaan 11 B-3, 2800, Mechelen, Belgium

    Lieven Baert

Authors
  1. Murali Pendela
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  2. Getu Weldu Kahsay
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  3. Guy Van den Mooter
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  4. Lieven Baert
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  5. Jos Hoogmartens
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  6. Erwin Adams
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Correspondence to Erwin Adams.

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Pendela, M., Kahsay, G.W., Van den Mooter, G. et al. LC Assay for a HIV Tablet Containing Emtricitabine, Tenofovir Disoproxil Fumarate and Rilpivirine. Chromatographia 73, 439–445 (2011). https://doi.org/10.1007/s10337-010-1906-1

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  • DOI: https://doi.org/10.1007/s10337-010-1906-1

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