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Separation and Determination of Amitriptyline and Nortriptyline in Biological Samples Using Single-Drop Microextraction with GC

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Abstract

The combination of liquid phase microextraction (LPME) based on a single drop and gas chromatography flame ionization detector (GC-FID) was used for separation and determination of amitriptyline and nortriptyline in human plasma and urine samples. The sample solution was kept alkaline (pH 12), then a microdrop of organic solvent (isooctane) was suspended in the donor solution; after extraction, the organic microdrop was injected into the GC-FID. Experimental LPME conditions were optimized. Finally, the enrichment factors (89.5–139.0), the relative standard deviation (RSD%, n = 5) 1.1–8.5, linearity ranges (0.05–20 μg mL−1), and the limits of detections (0.01, 0.02 μg mL−1) for selected drugs were evaluated.

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Acknowledgments

This work was financially supported by Ferdowsi University of Mashhad, Iran. The authors are grateful to Ms A. Beyg for providing us with the biological samples and Dr. A. Nateghi for the donation of standard materials. Also the kind help of Dr. Z. Es’haghi with studying this manuscript is appreciated.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Azadi Sq., Mashhad, Khorasan Razavi, Iran

    Ali Sarafraz Yazdi & Nourolhoda Razavi

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  1. Ali Sarafraz Yazdi
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  2. Nourolhoda Razavi
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Correspondence to Ali Sarafraz Yazdi.

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Sarafraz Yazdi, A., Razavi, N. Separation and Determination of Amitriptyline and Nortriptyline in Biological Samples Using Single-Drop Microextraction with GC. Chromatographia 73, 549–557 (2011). https://doi.org/10.1007/s10337-010-1900-7

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  • DOI: https://doi.org/10.1007/s10337-010-1900-7

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