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Synthesis and Evaluation of a Molecularly Imprinted Polymer for Pre-concentration of Patulin from Apple Juice

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Abstract

Patulin, (4-hydroxy-4H-furo[3,2-c] pyran-2(6H)-one) is a toxic secondary metabolite produced by a wide range of fungal species growing on some fruits, including apples. A novel molecularly imprinted polymer (MIP) for patulin has been synthesized using oxindole as a dummy template. The synthesis of MIPs based on dummy templates is a solution to overcome “template bleeding” shortcoming in trace analysis. The polymer was prepared in a non-covalent approach with methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker via free radical polymerization. It was revealed that the MIP particles in extraction columns displayed high affinity towards patulin and, therefore, subsequently employed for molecularly imprinted-solid phase extraction (MIP-SPE) of patulin from contaminated apple juice samples. The analysis of spiked samples showed good recoveries (>80%). Reproducibility, repeatability, and limit of detection of the proposed method were also investigated.

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Acknowledgments

This study was financially supported by the Islamic Azad University, Karaj Branch.

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Correspondence to Afshin Rajabi Khorrami.

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Khorrami, A.R., Taherkhani, M. Synthesis and Evaluation of a Molecularly Imprinted Polymer for Pre-concentration of Patulin from Apple Juice. Chromatographia 73 (Suppl 1), 151–156 (2011). https://doi.org/10.1007/s10337-010-1892-3

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  • DOI: https://doi.org/10.1007/s10337-010-1892-3

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