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An experimental test in Mallards (Anas platyrhynchos) of the effect of incubation and maternal preen oil on eggshell microbial load

Abstract

Microbial infection is one of the main factors reducing survival in the first stages of life in oviparous species, and recent studies have shown that the avian eggshell harbors an important variety of microorganisms that can rapidly multiply and penetrate the shell, leading to a decrease in hatchability. Here, we report the results of an experiment in which we examined how incubation and maternal preen oil affect the growth of avian eggshell microbes, using the Mallard (Anas platyrhynchos) as a model species. We compared the bacterial and fungal loads on the shell of non-incubated eggs and eggs incubated by females having free or blocked access to their preen gland. An increase of eggshell bacterial loads was observed in all conditions, but bacterial growth was higher on the shell of incubated eggs than on non-incubated eggs. We did not find any significant difference in eggshell bacterial growth for eggs incubated by females with free or blocked access to their preen gland. In addition, fungal growth during our experiment was not affected by incubation or the mother’s preen oil. Our findings are in contrast with those of previous studies which showed that incubation limited or had no effect on eggshell bacterial growth. Differences in environmental conditions and/or species ecology may explain the difference between the results of our experiment and those of previous studies. Our study provides the first data on the effect of maternal preen oil on eggshell microorganisms, showing that preen oil does not limit eggshell microbial growth.

Zusammenfassung

Ein experimenteller Test an Stockenten ( Anas platyrhynchos ) zum Effekt von Bebrütung und mütterlichem Bürzeldrüsenfett auf die Mikrobenbelastung von Eierschalen

Mikrobielle Infektionen sind ein Hauptfaktor für reduzierte Überlebensraten der ersten Lebensstadien bei oviparen Arten. Aktuelle Studien belegen, dass bei Vögeln die Eierschale eine erhebliche Vielfalt an Mikroorganismen beherbergt, die sich schnell vermehren und in die Schale eindringen können. Dies führt zu einer verringerten Schlupfrate. Hier stellen wir die Ergebnisse eines Experimentes vor, bei dem wir untersucht haben, wie Bebrütung und mütterliches Bürzeldrüsenfett das Wachstum von Mikroben auf Eierschalen von Vögeln beeinflussen. Als Modellart diente die Stockente (Anas platyrhynchos). Wir verglichen den Bakterien- und Pilzbefall der Schalen bebrüteter und nicht-bebrüteter Eier mittels Weibchen, die einen freien oder blockierten Zugriff auf ihre Bürzeldrüse hatten. Bei allen Eierschalen wurde ein Anstieg der Bakterienbelastung beobachtet, wobei das Bakterienwachstum auf Schalen bebrüteter Eier höher war als auf den Schalen unbebrüteter Eier. Hinsichtlich der Weibchen mit freiem oder blockiertem Zugriff auf ihre Bürzeldrüse konnte kein signifikanter Unterschied im Bakterienwachstum auf den Eierschalen festgestellt werden. Darüber hinaus war das Pilzwachstum während des Experimentes nicht beeinflusst durch die Bebrütung oder das Bürzeldrüsensekret des Weibchens. Unsere Ergebnisse stehen im Gegensatz zu früheren Studien, die gezeigt haben, dass die Bebrütung entweder einen limitierten oder gar keinen Effekt auf das Bakterienwachstum auf Eierschalen hatte. Unterschiede in den Umweltbedingungen und/oder der Ökologie der Arten könnten den Unterschied zwischen diesem Experiment und vorherigen Studien erklären. Die vorliegende Studie liefert die ersten Daten zum Einfluss von mütterlichem Bürzeldrüsensekret auf Mikroorganismen von Eierschalen, die zudem zeigen, dass das Bürzeldrüsensekret das mikrobielle Wachstum auf Eierschalen nicht begrenzt.

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Acknowledgments

We are grateful to Erika Hartmann, Nadine and Noël Guillon and Leopold Denoufoux for their help during field and laboratory work. We also thank Edward H. Burtt, Jr. and Steven R. Beissinger for their helpful comments. This project was partly supported by a French research grant from the ANR to Philipp Heeb (ANR-05, NT05-3_42075). MG was supported by a PhD scholarship of the French Government and GAC by an EGIDE grant.

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Giraudeau, M., Czirják, G.Á., Duval, C. et al. An experimental test in Mallards (Anas platyrhynchos) of the effect of incubation and maternal preen oil on eggshell microbial load. J Ornithol 155, 671–677 (2014). https://doi.org/10.1007/s10336-014-1050-z

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  • DOI: https://doi.org/10.1007/s10336-014-1050-z

Keywords

  • Preen oil
  • Incubation
  • Eggshell bacteria
  • Anas platyrhynchos