Quality assurance of PASADENA hyperpolarization for 13C biomolecules

Abstract

Object

Define MR quality assurance procedures for maximal PASADENA hyperpolarization of a biological 13C molecular imaging reagent.

Materials and methods

An automated PASADENA polarizer and a parahydrogen generator were installed. 13C enriched hydroxyethyl acrylate, 1-13C, 2,3,3-d3 (HEA), was converted to hyperpolarized hydroxyethyl propionate, 1-13C, 2,3,3-d3 (HEP) and fumaric acid, 1-13C, 2,3-d2 (FUM) to hyperpolarized succinic acid, 1-13C, 2,3-d2 (SUC), by reaction with parahydrogen and norbornadiene rhodium catalyst. Incremental optimization of successive steps in PASADENA was implemented. MR spectra and in vivo images of hyperpolarized 13C imaging agents were acquired at 1.5 and 4.7 T.

Results

Application of quality assurance (QA) criteria resulted in incremental optimization of the individual steps in PASADENA implementation. Optimal hyperpolarization of HEP of P = 20% was achieved by calibration of the NMR unit of the polarizer (B 0 field strength ± 0.002 mT). Mean hyperpolarization of SUC, P = [15.3 ± 1.9]% (N = 16) in D 2O, and P = [12.8 ± 3.1]% (N = 12) in H 2O, was achieved every 5–8 min (range 13–20%). An in vivo 13C succinate image of a rat was produced.

Conclusion

PASADENA spin hyperpolarization of SUC to 15.3% in average was demonstrated (37,400 fold signal enhancement at 4.7 T). The biological fate of 13C succinate, a normally occurring cellular intermediate, might be monitored with enhanced sensitivity.

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Correspondence to Brian D. Ross.

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Hövener, JB., Chekmenev, E.Y., Harris, K.C. et al. Quality assurance of PASADENA hyperpolarization for 13C biomolecules. Magn Reson Mater Phy 22, 123–134 (2009). https://doi.org/10.1007/s10334-008-0154-y

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Keywords

  • Hyperpolarization
  • PASADENA
  • Fumarate
  • Succinate
  • Parahydrogen
  • 13C MRI