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Monitoring demyelination and remyelination by magnetization transfer imaging in the mouse brain at 9.4 T

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Abstract

Objective

The aim of this study was to assess quantitatively structural changes in myelin content occurring during demyelination and remyelination by magnetization transfer imaging (MTI).

Materials and methods

In a reversible model of demyelination with no axonal loss, mice intoxicated by cuprizone were studied by MTI in vivo at 9.4 T. MRI data were compared to histopathological examinations.

Results

Data revealed that the magnetization transfer ratio (MTR) decreased significantly during demyelination and increased during remyelination with strong correlation to the myelin content (r = 0.79, P = 0.01).

Conclusions

This study demonstrated that MTR is a sensitive and reproducible quantitative marker to assess myelin loss and repair. This may lead to in vivo monitoring of therapeutic strategies promoting remyelination.

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Authors and Affiliations

  1. Résonance Magnétique des Systèmes Biologiques, UMR5536 CNRS/Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat Case 93, 33076, Bordeaux Cedex, France

    Wafaa Zaaraoui, Michel Merle, Gérard Raffard, Marc Biran & Jean-Michel Franconi

  2. Laboratoire de Neurobiologie des Affections de la Myéline, EA 2966, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076, Bordeaux Cedex, France

    Mathilde Deloire, Céline Girard, Klaus G. Petry, Bruno Brochet & Vincent Dousset

  3. Department of Radiology, New York University School of Medicine, 650 First Avenue, New York, 10016, USA

    Matilde Inglese & Oded Gonen

Authors
  1. Wafaa Zaaraoui
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  2. Mathilde Deloire
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  3. Michel Merle
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  4. Céline Girard
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  5. Gérard Raffard
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  6. Marc Biran
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  7. Matilde Inglese
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  8. Klaus G. Petry
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  9. Oded Gonen
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  10. Bruno Brochet
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  11. Jean-Michel Franconi
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  12. Vincent Dousset
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Corresponding author

Correspondence to Wafaa Zaaraoui.

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Zaaraoui, W., Deloire, M., Merle, M. et al. Monitoring demyelination and remyelination by magnetization transfer imaging in the mouse brain at 9.4 T. Magn Reson Mater Phy 21, 357–362 (2008). https://doi.org/10.1007/s10334-008-0141-3

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  • DOI: https://doi.org/10.1007/s10334-008-0141-3

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