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1H NMR-based metabolomic identification of at-risk areas after myocardial infarction in swine

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Abstract

Object

1H NMR-based metabolic profiling has been used to investigate areas of the heart after an acute myocardial infarction.

Methods

Tissue was obtained from control, at-risk (areas that survive within the infarct zone) and necrotic myocardium after 48 min of left anterior descending coronary artery occlusion and 2 h of reperfusion in a swine model. HR-MAS (high resolution magic angle spectroscopy) spectra from intact tissue and tissue extract spectra were obtained for each region and statistical models were built for each type of spectra allowing differentiation between control, at-risk and necrotic heart.

Results

At-risk and, especially, necrotic areas have a reduced concentration of NMR visible metabolites as compared to control tissue, total creatine (phosphorilated and unphosphorilated) being the single most important metabolite in the different discriminant models. Creatine concentration decreased from 18.28  ±  0.84 μmols/g fresh weight in controls to 12.58  ±  2.89 (P < 0.05) and 9.96  ±  2.21 (P < 0.01) in at-risk and necrotic areas, respectively. Taurine and myo-inositol were also involved in the discriminant models. HR-MAS spectra also showed an increase in lipid signals at 0.9 and 1.28 ppm as markers of necrotic tissue. These results support the view that the analysis of in vivo 1H MRS may have value in differentiating normal, at-risk and infarcted myocardium.

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Abbreviations

LAD:

Left anterior descending coronary artery

PCs:

Principal component(s)

PCA:

Principal component analysis

PLS-DA:

Partial least squares discriminant analysis

VIP:

Variable influence on projection

HR-MAS:

High resolution magic angle spinning

BSA:

Bovine serum albumin

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Authors and Affiliations

  1. Laboratori de Cardiologia Experimental, Servei de Cardiologia, Hospital Universitari Vall d′Hebron, Pg. Vall d′Hebron 129, 08035, Barcelona, Spain

    Ignasi Barba, Ester Jaimez-Auguets, Antonio Rodriguez-Sinovas & David Garcia-Dorado

Authors
  1. Ignasi Barba
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  2. Ester Jaimez-Auguets
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  3. Antonio Rodriguez-Sinovas
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  4. David Garcia-Dorado
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Corresponding author

Correspondence to David Garcia-Dorado.

Additional information

This work was supported by Red de Enfermedades Cardiovasculares (RECAVA) CICYT SAF2005-01758 and EU Grant FP6-513595.

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Barba, I., Jaimez-Auguets, E., Rodriguez-Sinovas, A. et al. 1H NMR-based metabolomic identification of at-risk areas after myocardial infarction in swine. Magn Reson Mater Phy 20, 265–271 (2007). https://doi.org/10.1007/s10334-007-0097-8

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  • DOI: https://doi.org/10.1007/s10334-007-0097-8

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