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Dynamic contrast-enhanced MRI using macromolecular contrast media for monitoring the response to isolated limb perfusion in experimental soft-tissue sarcomas

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Abstract

The objective of this study was to evaluate the potential of dynamic contrast-enhanced MRI for quantitative characterization of tumor microvessels and to assess the microvascular changes in response to isolated limb perfusion with TNF-α and melphalan. Dynamic contrast-enhanced MRI was performed in an experimental cancer model, using a macromolecular contrast medium, albumin-(Gd-DTPA)45. Small fragments of BN 175, a soft-tissue sarcoma, were implanted in 11 brown Norway (BN) rats. Animals were assigned randomly to a control (Haemaccel) or drug-treated group (TNF-α/melphalan). MRI was performed at baseline and 24 h after ILP. The transendothelial permeability (KPS) and the fractional plasma volume (fPV) were estimated from the kinetic analysis of MR data using a two-compartment bi-directional model. KPS and fPV decreased significantly in the drug-treated group compared to baseline (p<0.05). In addition, KPS post therapy was significantly lower (p<0.05) in the drug-treated group than in the control group. There was no significant difference in fPV between the drug-treated and the control group after therapy. Tumor microvascular changes in response to isolated limb perfusion can be determined after 24 h by dynamic contrast-enhanced MRI. The data obtained in this experimental model suggest possible applications in the clinical setting, using the appropriate MR contrast agents.

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Acknowledgments.

Albumin-(Gd-DTPA)45 was generously provided by RC Brasch, Center of Pharmaceutical and Molecular Imaging, Department of Radiology, University of California San Francisco, CA, USA.

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Authors and Affiliations

  1. Department of Radiology, Erasmus MC-University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015, GD, Rotterdam, The Netherlands

    A. Preda, P. A. Wielopolski, M. van Vliet, J. F. Veenland, M. W. Vogel, G. P. Krestin & C. F. van Dijke

  2. Department of Surgical Oncology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands

    T. L. M. ten Hagen, G. Ambagtsheer, S. T. van Tiel & A. M. M. Eggermont

Authors
  1. A. Preda
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  2. P. A. Wielopolski
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  3. T. L. M. ten Hagen
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  4. M. van Vliet
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  5. J. F. Veenland
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  6. G. Ambagtsheer
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  7. S. T. van Tiel
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  8. M. W. Vogel
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  9. A. M. M. Eggermont
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  10. G. P. Krestin
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  11. C. F. van Dijke
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Correspondence to A. Preda.

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Preda, A., Wielopolski, P., Hagen, T. et al. Dynamic contrast-enhanced MRI using macromolecular contrast media for monitoring the response to isolated limb perfusion in experimental soft-tissue sarcomas. MAGMA 17, 296–302 (2004). https://doi.org/10.1007/s10334-004-0050-z

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  • DOI: https://doi.org/10.1007/s10334-004-0050-z

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