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Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events

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Abstract

In seed priming, seeds are soaked in a solution of low water potential and dried back to their original weight. This study was conducted to evaluate the comparative performance of different seed priming treatments and to investigate whether re-drying is essential or not? Seeds of fine grain aromatic rice (Oryza sativa L.) cultivar Super-Basmati were subjected to hardening, osmohardening with CaCl2 and KCl (ψ s −1.25 MPa) each for 24 h (one cycle), and hydropriming and ascorbate priming (10 mg L−1) for 48 h. Seeds were primed in two sets. In one set after each treatment, seeds were given three surface washings with distilled water and dried back near to original moisture contents with forced air. In the other case, after surface washings with distilled water, seeds were surface dried using blotting paper and sown immediately. All the priming treatments improved the emergence, seedling growth and reserve metabolism. Use of surface drying was more effective for rice seed invigoration, as evident from earlier and synchronized seedling emergence. Moreover, shoot and root length, seedling dry weight, root score, α-amylase activity, soluble sugars and dehydrogenase activity were also improved, although germination percentage and leaf score were similar from both strategies. Results suggested that surface drying, rather than re-drying close to original weight, was more effective, while among the treatments, osmohardening with CaCl2 was the most effective. Increasing rice production with judicious use of water is need of the day, and aerobic rice cultivation is an attractive alternative for this purpose. However, poor and erratic stand establishment is one of the major hindrances in its wide scale adoption. In this regard, seed priming techniques are pragmatic approaches to achieve proper stand establishment in the new rice culture. They help in improving seedling density per unit area under optimal and adverse soil conditions and may be opted to improve the performance of aerobic rice.

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References

  • Afzal I, Basra SMA, Shahid M, Farooq M, Saleem M (2008) Priming enhances germination of spring maize (Zea mays L.) under cool conditions. Seed Sci Technol 36:497–503

    Google Scholar 

  • Association of Official Seed Analysis (1983) Seed vigor testing handbook. Contribution No. 32 to the handbook on seed testing. Association of Official Seed Analysis. Springfield, IL

  • Basra SMA, Farooq M, Tabassum R (2005) Physiological and biochemical aspects of seed vigour enhancement treatments in fine rice (Oryza sativa L.). Seed Sci Technol 33:623–628

    Google Scholar 

  • Basra SMA, Farooq M, Wahid A, Khan MB (2006a) Rice seed invigoration by hormonal and vitamin priming. Seed Sci Technol 34:775–780

    Google Scholar 

  • Basra SMA, Farooq M, Tabassum R, Ahmad N (2006b) Evaluation of seed vigor enhancement techniques on physiological and biochemical basis in coarse rice. Seed Sci Technol 34:741–750

    Google Scholar 

  • Bernfeld P (1955) In: Cdowick SP, Kaplan I (eds) Amylases α and β; methods in enzymology, vol 1. Academic Press, New York, p 149

  • Bewley JD, Black M (1985) Seed physiology of development and germination. Plenum, New York

    Google Scholar 

  • Bruggink GT, Ooms JJJ, van der Toornm P (1999) Induction of longevity in primed seeds. Seed Sci Res 9:49–53

    Google Scholar 

  • Chiu KY, Chen CL, Sung JM (2002) Effect of priming temperature on storability of primed sh-2 sweet corn seed. Crop Sci 42:1996–2003

    CAS  Google Scholar 

  • Coolbear P, Francis A, Grierson D (1984) The effect of low temperature pre-sowing treatment under the germination performance and membrane integrity of artificially aged tomato seeds. J Exp Bot 35:1609–16170

    Article  CAS  Google Scholar 

  • Davison PA, Bray CM (1991) Changes in ribosomal RNA integrity in leek (Allium porrum L.) seeds during osmoconditioning and drying-back treatments. Seed Sci Res 1:37–44

    CAS  Google Scholar 

  • Dearman J, Brocklehust PA, Drew RLK (1987) Effect of osmotic priming and aging on the germination and emergence of carrot and leek seed. Ann Appl Biol 111:717–722

    Article  Google Scholar 

  • Dell’Aquilla A, Spada P (1992) Regulation of protein synthesis in germinating wheat embryos under polyethylene glycol and salt stress. Seed Sci Res 2:75–80

    Google Scholar 

  • Dell’Aquilla A, Tritto V (1991) Germination and biochemical activities in wheat seeds following delayed harvesting, ageing and osmotic priming. Seed Sci Technol 19:73–82

    Google Scholar 

  • Dubois M, Giles KA, Hamilton JK, Roberes PA, Smith F (1956) Colorometric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Ellis RA, Roberts EH (1981) The quantification of ageing and survival in orthodox seeds. Seed Sci Technol 9:373–409

    Google Scholar 

  • Farooq M, Basra SMA, Ahmad N, Hafeez K (2005) Thermal hardening: a new seed vigour enhancement tool in rice. J Integr Plant Biol 47:187–193

    Article  Google Scholar 

  • Farooq M, Basra SMA, Hafeez K (2006a) Seed invigoration by osmohardening in coarse and fine rice. Seed Sci Technol 34:181–187

    Google Scholar 

  • Farooq M, Basra SMA, Afzal I, Khaliq A (2006b) Optimization of hydropriming techniques for rice seed invigoration. Seed Sci Technol 34:507–512

    Google Scholar 

  • Farooq M, Basra SMA, Wahid A (2006c) Priming of field-sown rice seed enhances germination, seedling establishment, allometry and yield. Plant Growth Regul 49:285–294

    Article  CAS  Google Scholar 

  • Farooq M, Basra SMA, Tabassum R, Afzal I (2006d) Enhancing the performance of direct seeded fine rice by seed priming. Plant Prod Sci 9:446–456

    Article  Google Scholar 

  • Farooq M, Basra SMA, Khalid M, Tabassum R, Mehmood T (2006e) Nutrient homeostasis, reserves metabolism and seedling vigor as affected by seed priming in coarse rice. Can J Bot 84:1196–1202

    Article  CAS  Google Scholar 

  • Farooq M, Basra SMA, Ahmad N (2007a) Improving the performance of transplanted rice by seed priming. Plant Growth Regul 51:129–137

    Article  CAS  Google Scholar 

  • Farooq M, Basra SMA, Khan MB (2007b) Seed priming improves growth of nursery seedlings and yield of transplanted rice. Arch Agron Soil Sci 53:315–326

    Article  Google Scholar 

  • Farooq M, Aziz T, Basra SMA, Cheema MA, Rehman H (2008a) Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. J Agron Crop Sci 194:161–168

    Article  CAS  Google Scholar 

  • Farooq M, Aziz T, Basra SMA, Cheema MA, Rehman H (2008b) Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. J Agron Crop Sci 194:161–168

    Article  CAS  Google Scholar 

  • Farooq M, Aziz T, Hussain M, Rehman H, Jabran K, Khan MB (2008c) Glycinebetaine improves chilling tolerance in hybrid maize. J Agron Crop Sci 194:152–160

    Article  CAS  Google Scholar 

  • Farooq M, Kobayashi N, Wahid A, Ito O, Basra SMA (2009a) Strategies for producing more rice with less water. Adv Agron 101:351–388

    Article  Google Scholar 

  • Farooq M, Basra SMA, Wahid A, Khaliq A, Kobayashi N (2009b) Rice seed invigoration. In: Lichtfouse E (ed) Sustainable agriculture reviews—book series. Springer, The Netherlands

    Google Scholar 

  • Fu JR, Lu XH, Chen RZ, Zhang BZ, Liu ZS, Ki ZS, Cai CY (1988) Osmoconditioning of peanut (Arachis hypgaea L.) seeds with PEG to improve vigor and some biochemical activities. Seed Sci Technol 16:197–212

    CAS  Google Scholar 

  • Giri GS, Schillinger WF (2003) Seed priming winter wheat for germination, emergence and yield. Crop Sci 43:2135–2141

    Article  Google Scholar 

  • Goel A, Goel AK, Sheoran IS (2003) Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J Plant Physiol 160:1093–1100

    Article  CAS  PubMed  Google Scholar 

  • Harris D, Joshi A, Khan PA, Gothkar P, Sodhi PS (1999) On-farm seed priming in semi-arid agriculture: development and evaluation in maize, rice and chickpea in India using participatory methods. Exp Agr 35:15–29

    Article  Google Scholar 

  • Harris D, Tripathi RS, Joshi A (2002) On-farm seed priming to improve crop establishment and yield in dry direct-seeded rice. In: Pandey S, Mortimer M, Wade L, Tuong TP, Lopes K, Hardy B (eds) Direct seeding: research strategies and opportunities. International Research Institute, Los Baños, Philippines, pp 231–240

    Google Scholar 

  • Heydecker W, Higgins J, Gulliver RL (1973) Accelerated germination by osmotic seed treatment. Nature 246:42–46

    Article  CAS  Google Scholar 

  • Kittock DL, Law AG (1968) Relationship of seedling vigour, respiration, and tetrazolium chloride reduction by germinating wheat seeds. Agron J 60:286–288

    CAS  Google Scholar 

  • Koehler KH, Voigt B, Spittler H, Schelenz M (1997) Biochemical events after priming and osmoconditioning of seeds. In: Ellis RH, Black M, Murdoch AJ, Hong TD (eds) Basic and Applied aspects of seed biology. Proceedings of fifth international workshop on seeds, Reading, pp 531–536

  • Kulkarni GN, Eshanna MR (1988) Effect of pre-soaking of corn seed on seed quality. Seed Res 16:37–40

    Google Scholar 

  • Lee SS, Kim JH (2000) Total sugars, α-amylase activity, and emergence after priming of normal and aged rice seeds. Kor J Crop Sci 45:108–111

    Google Scholar 

  • Lee SS, Kim JH, Hong SB, Yun SH (1998) Effect of humidification and hardening treatment on seed germination of rice. Kor J Crop Sci 43:157–160

    Google Scholar 

  • Murungu FS, Chiduza C, Nyamugafata P, Clark LJ, Whalley WR, Finch-Savage WE (2004) Effects of ‘on-farm seed priming’ on consecutive daily sowing occasions on the emergence and growth of maize in semi-arid Zimbabwe. Field Crops Res 89:49–57

    Article  Google Scholar 

  • Parera CA, Cantliffe DJ (1994) Pre-sowing seed priming. HortRev 16:109–141

    Google Scholar 

  • Paul SR, Choudhary AK (1991) Effect of seed priming with salts on growth and yield of wheat under rainfed conditions. Ann Agr Res 12:415–418

    Google Scholar 

  • Smith PT, Cobb BG (1991) Accelerated germination of pepper seed by priming with salt solutions and water. HortSci 26:417–419

    CAS  Google Scholar 

  • Srivastava LM (2002) Plant growth and development: hormones and environment. Academic Press, London, p 240

    Google Scholar 

  • Sung FJ, Chang YH (1993) Biochemical activities associated with priming of sweet corn seeds to improve vigor. Seed Sci Technol 21:97–105

    Google Scholar 

  • Taiz L, Zeiger E (2006) Plant physiology, 4th edn. Sinauer Associates Inc., Massachusetts, p 764

    Google Scholar 

  • Tuong TP, Bouman BAM (2002) Rice production in water-scarce environments. Paper presented at the water productivity workshop, Colombo, Sri Lanka, 12–14 Nov 2001

  • Wahid A, Saher S, Perveen M, Gelani S, Basra SMA, Farooq M (2008a) Seed pretreatment with hydrogen peroxide improves heat tolerance in maize at germination and seedling growth stages. Seed Sci Technol 36:633–645

    Google Scholar 

  • Wahid A, Noreen S, Basra SMA, Gelani S, Farooq M (2008b) Priming-induced metabolic changes in sunflower (Helianthus annuus) achenes improve germination and seedling growth. Bot Stud 49:343–350

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Agronomy, University of Agriculture, Faisalabad, 38040, Pakistan

    Muhammad Farooq

  2. Department of Botany, University of Agriculture, Faisalabad, 38040, Pakistan

    Abdul Wahid

  3. Department of Crop Physiology, University of Agriculture, Faisalabad, 38040, Pakistan

    Nazir Ahmad

  4. Division of Agricultural and Environmental Sciences, School of Biosciences, University of Nottingham, Nottingham, NG7 2RD, UK

    Saeed A. Asad

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  1. Muhammad Farooq
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  2. Abdul Wahid
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  3. Nazir Ahmad
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  4. Saeed A. Asad
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Correspondence to Muhammad Farooq.

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Farooq, M., Wahid, A., Ahmad, N. et al. Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events. Paddy Water Environ 8, 15–22 (2010). https://doi.org/10.1007/s10333-009-0170-1

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