Skip to main content
SpringerLink
Log in

Construction the hairpin RNA recombinant plasmids targeting human Pokemon gene

  • Published:
The Chinese-German Journal of Clinical Oncology

Abstract

Objective

The aim of this study was to establish the foundation for studying the role of pokemon gene in tumorigenesis and development by constructing recombinant plasmids that can express small interfering RNA (siRNA) targeting human Pokemon gene.

Methods

Hairpin siRNA templates targeting Pokemon gene were synthesized and cloned into plasmid vector psiRNA-H1neo. Three vectors derived siRNAs (psiRNA1, 2, 3) and one mocking psiRNAc (as control) were constructed. The recombinant Pokemon siRNA plasmids were constructed and identified using restrictive enzyme analysis and DNA sequencing.

Results

Restrictive enzyme analysis and DNA sequencing revealed the successful construction of siRNA expression plasmids.

Conclusion

Constructing siRNA templates targeting Pokemon gene may provide us with practical tools for further study the role of Pokemon gene in the development of some diseases and gene therapy of tumor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Maeda T, Hobbs RM, Merghoub T, et al. Role of the proto-oncogene Pokemon in cellular transformation and ARF repression. Nature, 2005, 433: 278–285.

    Article  PubMed  CAS  Google Scholar 

  2. Maeda T, Hobbs RM, Pandolfi PP. The transcription factor Pokemon: a new key player in cancer pathogenesis. Cancer Res, 2005, 65: 8575–8578.

    Article  PubMed  CAS  Google Scholar 

  3. Reynolds A, Leake D, Boese Q, et al. Rational siRNA design for RNA interference. Nat Biotechnol, 2004, 22: 326–330.

    Article  PubMed  CAS  Google Scholar 

  4. Schubert S, Grunweller A, Erdmann V A, et al. Local RNA target structure influences siRNA efficacy: systematic analysis of intentionally designed binding regions. J Mol Biol, 2005, 348: 883–893.

    Article  PubMed  CAS  Google Scholar 

  5. Fire A, Xu S, Montgomery MK, et al. Potent and sepecific genetic interference by doule-stranded RNA in caenorhabditis elegans. Nature, 1998, 391: 806.

    Article  PubMed  CAS  Google Scholar 

  6. Bernstein E, Denli AM, Hannon GJ. The rest is silence. RNA, 2001, 7: 1509.

    PubMed  CAS  Google Scholar 

  7. Scherr M, Morgan MA, Eder M. Gene silencing mediated by small interfering RNAs in mammalian cells. Curr Med Chem, 2003, 10: 245–256.

    PubMed  CAS  Google Scholar 

  8. Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science, 2002, 296: 550–553.

    Article  PubMed  CAS  Google Scholar 

  9. Lu B, Mu Y, Cao C, et al. Survivin as a therapeutic target for radiation sensitization in lung cance. Cancer Res, 2004, 64: 2840–2845.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oncology, Northern Jiangsu People’s Hospital, Clinical Medical School, Yangzhou University, Yangzhou, 225001, China

    Wenmiao Cao, Buhai Wang & Xizhi Zhang

  2. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430023, China

    Wenmiao Cao, Tao Zhang, Jinghua Ren & Jie Yao

Authors
  1. Wenmiao Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Buhai Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xizhi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jinghua Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jie Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xizhi Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cao, W., Zhang, T., Wang, B. et al. Construction the hairpin RNA recombinant plasmids targeting human Pokemon gene. Chin. -Ger. J. Clin. Oncol. 10, 730–733 (2011). https://doi.org/10.1007/s10330-011-0876-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10330-011-0876-6

Key words

Search

Navigation