Abstract
Objective
The aim of the study was to investigate the mechanism of gemcitabine (GEM) combination with radiation on the high metastasis human ovarian cancer cell line (HO-8910PM).
Methods
Human ovarian cancer cell line HO-8910PM was treated with different concentrations of gemcitabine for 24 h, then the cells were counted. In the study of GEM combination with radiation, an efficiency of colony formation was observed; the cell cycle and apoptosis were analyzed by flow cytometry; the experiment of depend on the time and its radio sensitivity were observed by using mitotic index with the cells for each 24, 48, 72 and 96 h after experiment.
Results
It suggested that the GEM had an inhibition effect on the human ovarian cancer cell line. The alive cell numbers were decreased by following a height of GEM concentration. When GEM in combination with a radiation, the suppression was significantly increased than that of single GEM therapy. The efficiency of colony formation was significantly lower, under this condition the cell could be arrested at G0–G1 phase and could be decreased to enter into the S phase; the apoptosis percentage could be significantly increased; especially, under the 4 Gy and 6 Gy doses the cell apoptosis was more obvious. GEM combination with radiation had depended on the time to the cells; mitotic index of the cells in combination group was observed significantly lower than that of single GEM therapy or single radiation, and this showed that it had an effect of radiosensitivity.
Conclusion
The GEM has a significant growth inhibition on the human ovarian cancer cells, GEM combination with radiation could induce HO-8910PM cell occurred arrested and apoptosis. It has depended on the time and has a radiosensitivity effect. The result shows that it is a better method to treat the human ovarian cancer by using radiotherapy combined with gemcitabine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Zhou XY, Zhu M, Wang JD, et al. Treatment of primary liver cancer with postoperative after-loading radiotherapy and gemcitabine. Chinese-German J Clin Oncol, 2005, 4: 203–205.
Xu L, Luo RC. The clinical study of taxol and gemcitabine for treating the advanced breast cancer combination with cisplatin respectively. Chongqing Med J (Chinese), 2005, 34: 440–441.
Jiang H, Fan GH, Li QZ, et al. The synergetic effect of ginsenoside and gemcitabine on human lung cancer cells derived from pleural effusion. J Nanhua Univ (Med Edit) (Chinese), 2002, 30: 7–10.
Pauwels B, Korst AEC, Lardon F, et al. Combined modality therapy of gemcitabine and radiation. The Oncologist, 2005, 10: 34–51.
Bernstein ED, Herbert SM, Hanna NH. Chemotherapy and radiotherapy in the treatment of resectable non-small-cell lung cancer. Ann Surg Oncol, 2006, 13: 291–301.
Li CL, Hou M. Advance in the study of the radiosensitivity of gemcitabine. Chin J Lung Cancer (Chinese), 2002, 5: 319–320.
Li SJ, Jiang JJ, Bai CX. Experimental study on improving radiosensitivity for Lewis lung cancer with gemcitabine. Sect Respir Sys Foreign Med Sci (Chinese), 2005, 25: 84–87.
Miao JW, Kong WM, Zhang WH, et al. Mechanistic study of radiosensitization by gemcitabine on human squamous carcinoma cell line of the cervix. Chin J Obstet Gynecol (Chinese), 2004, 39: 342–345.
Wu ZB, Ma SL. The research progress of the radiosensitizing effect of gemcitabine. Chin Clin Oncol (Chinese), 2004, 9: 200–201.
Murphy JD, Lucas DR, Somnay YR, et al. Gemcitabine-mediated radiosensitization of human soft tissue sarcoma. Transl Oncol, 2008, 1: 50–56.
Xu SH, Qian LJ, Mou HZ, et al. Establishment of the highly metastatic human ovarian cancer cell line (HO-8910PM) and its characterization. Exp Clin Cancer Res, 1999, 18: 233–239.
Shewach DS, Lawrence TS. Radiosensitization of human tumor cells by gemcitabine in vitro. Semin Oncol, 1995, 22: 68–71.
Choy H. Combination chemoradiotherapy with gemcitabine: potential applications. Oncology, 2000, 14: 20–25.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gu, L., Qian, L., Zhu, C. et al. Experimental study of gemcitabine combination with radiation in vitro on a highly metastatic human ovarian cancer cell line HO-8910PM. Chin. -Ger. J. Clin. Oncol. 9, 648–651 (2010). https://doi.org/10.1007/s10330-010-0701-7
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10330-010-0701-7