The effect of low dose radiation on cytokine correlated to injury of lungs induced by Bleomycin A5

Abstract

Objective

The aim of this study was to explore the effect of low dose radiation on cytokine excreted by mice inbreathing of atomization of PYM.

Methods

Kunming male mice were randomly divided into three groups: blank group, PYM group (P group), low dose radiation + PYM group (P + L group). Mice of P + L group were given whole body low dose radiation 75 mGY, dose rate were 12.5 mGY/min. After 6 h, mice in both P + L group and P group were given inbreathe of atomization of PYM, concentration was 2 mg/mL. Mice were sacrificed after the d1, d7, d14, d21 and d28, IL-6 were detected in alveolar irrigating solution. The tissue samples of mice lung were fixed in 10% formalin, TNF-α and TGF-β were analyzed by immunohistochemistry.

Results

Compared with P group, IL-6 in low dose radiation + PYM group were lower, near to blank group, the difference had notable statistical significance on the d1 and the d7, while on the d14, d21, d28, the difference had not statistical significance. It suggested that low dose radiation could reduce the resection of IL-6 at the begin of lung injure induced by low dose radiation. The expression of TGF-β and TNF-α in P + L group were lower than that in P group, but the difference in the two groups had statistical significance by gray analysis P < 0.05.

Conclusion

In the early stage of lung injure caused by PYM in mice, low dose radiation of 75 mGY can reduce the secretion of IL-6, decrease the production of TGF-β and TNF-α.