Abstract
Objective
We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation.
Methods
The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by liposome mediation for transient expression. After AP-2α transfected SW620 cells, the exogenous AP-2α mRNA and protein express were determined by the method of Real-time PCR and Western blot. In order to elucidate the effect of expression of exogenous AP-2α gene on the colorectal cancer cell SW620, the proliferation rates were analyzed by growth curves for cells including SW620, pcDNA3.1(+)/SW620 and pcDNA3.1(+)-AP-2α/SW620. At same time, the apoptotic rate of cells and the cell cycle analysis were also tested by flow cytometry.
Results
The mRNA and protein expressions of AP-2α gene could be enhanced by transfecting of pcDNA3.1(+)-AP-2α recombinant plasmid into SW620 cell. The cell growth rates of SW620 cells transfected with pcDNA3.1(+)/AP-2α were slower than those transfected with pcDNA3.1(+) or untransfected. The apoptotic rates were increased compared with pcDNA3.1(+)/SW620 and SW620 (P < 0.05), which indicated that over-expression of AP-2α gene could efficiently inhibit the growth of SW620 cells and induce apoptosis. FCM analyses indicated that the cells being arrested in G1 phase.
Conclusion
AP-2α gene can be efficiently transfected into SW620 cells and over-expression AP-2α protein in the transfected cells. AP-2α induces G1 arrest and apoptosis, suppressive effect on cell growth.
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Du, Y., Xu, L., Miao, J. et al. AP-2α gene transfection effects cell proliferation and cell apoptosis of SW620 cells line in colorectal carcinoma. Chin. -Ger. J. Clin. Oncol. 9, 221–225 (2010). https://doi.org/10.1007/s10330-010-0007-9
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DOI: https://doi.org/10.1007/s10330-010-0007-9