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Inhibitory effects of transfected Bcl-XL antisense oligodeoxynucleotide to proliferation and growth in esophageal carcinoma cell line and human esophageal carcinoma xenograft of nude mice

  • Published:
The Chinese-German Journal of Clinical Oncology

Abstract

Objective

The aim of our study was to investigate the biological effects of Bcl-XL antisense oligodeoxynucleotide (ASODN) transfected into cultured esophageal carcinoma cells and human esophageal carcinoma xenograft in nude mice.

Methods

Cationic liposome-mediated ASODN was used to transfect esophageal carcinoma cells. RT-PCR, Western blot, MTT assay, flow cytometry, and in situ apoptosis cells detection (TUNEL detection) were used to systematically study the biological effects of transfected cells both in vitro and in vivo.

Results

In this study, the results showed that the proliferation of esophageal carcinoma cells in ASODN group decreased significantly when compared with the control group (P < 0.05), at 57.3% Bcl-XL mRNA inhibitory rate, and a significant decreasing of Bcl-XL protein expression, at the apoptosis rates of (31.1 ± 5.8)% and 35.0% by flow cytometry and TUNEL assay respectively (P < 0.01, when compared with control groups). It also showed that the growth of human esophageal carcinoma in nude mice of ASODN group was significantly inhibited (P < 0.05), together with a significant decreased expression level of Bcl-XL mRNA and protein, and an induced tumor cell apoptosis in nude mice.

Conclusion

Our result indicates Bcl-XL ASODN can effectively inhibit the proliferation of esophageal carcinoma cells in vitro and tumor growth in vivo. The suppression of Bcl-XL expression by ASODN may offer both a therapeutic approach and an important theoretic foundation for gene therapy against esophageal carcinoma.

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Authors and Affiliations

  1. Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China

    Lei Zhang

  2. Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China

    Hongtao Wen

  3. Henan Key Laboratory of Tumor Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China

    Lan Zhang, Dongling Gao, Shenglei Li, Fengyu Cao, Kuisheng Chen & Yunhan Zhang

  4. Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China

    Lan Zhang, Dongling Gao, Shenglei Li, Fengyu Cao, Kuisheng Chen & Yunhan Zhang

Authors
  1. Lei Zhang
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  2. Hongtao Wen
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  3. Lan Zhang
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  4. Dongling Gao
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  5. Shenglei Li
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  6. Fengyu Cao
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  7. Kuisheng Chen
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  8. Yunhan Zhang
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Corresponding author

Correspondence to Lei Zhang.

Additional information

Supported by a grant from the Henan Innovation Project for University Prominent Research Talents (No. 2007KYCX005).

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Zhang, L., Wen, H., Zhang, L. et al. Inhibitory effects of transfected Bcl-XL antisense oligodeoxynucleotide to proliferation and growth in esophageal carcinoma cell line and human esophageal carcinoma xenograft of nude mice. Chin. -Ger. J. Clin. Oncol. 9, 169–174 (2010). https://doi.org/10.1007/s10330-010-0006-x

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  • DOI: https://doi.org/10.1007/s10330-010-0006-x

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