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Expression pattern of GATA-1, -2 and -3 genes in leukemic bone marrow microenvironment

  • Published:
The Chinese-German Journal of Clinical Oncology

Abstract

Objective

The aim of the study was to investigate the expression pattern of hematopoietic transcription factor GATA-1, -2 and -3 genes in leukemic bone marrow (BM) microenvironment [including bone marrow stromal cells (BMSCs) and BM hematopoietic cells].

Methods

Mononuclear cells were isolated from BM of patients with acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), or acute lymphoblastic leukemia (ALL). Adherent cells (BMSCs) and nonadherent cells (BM hematopoietic cells) were collected after long-term culture in vitro. The semi-quantitative expression levels of GATA genes in the BMSCs or BM hematopoietic cells from patients with leukemia were analyzed by using RT-PCR-ELISA and compared with normal controls.

Results

The expression level of GATA-1 gene in the BMSCs from CML group was significantly lower than that of the normal controls. The expression level of GATA-3 gene in the BMSCs from ALL was higher than that of the normal controls, but that from CML was lower than the normal controls. Dominant expression of GATA-3 gene was found in the normal BM hematopoietic cells. The dominant expression of GATA-2 gene was found in the normal BMSCs and the BMSCs from CML, whereas the dominant expression of GATA-3 gene was detected in the BMSCs from AML.

Conclusion

GATA-1, -2 and -3 genes might play a role in hematopoiesis regulation in leukemia, and the changes of expression pattern of GATA genes might influence the hematopoiesis in BM microenvironment and relate to the pathogenesis and development of leukemia.

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Authors and Affiliations

  1. Institute of Hematology, Medical College of Jinan University, Guangzhou, 510632, China

    Xiuli Wu, Yangqiu Li, Kanger Zhu, Zhen Wang, Shaohua Chen & Lijian Yang

  2. Institute of Hematology, Chinese Academy of Medical Sciences and the Peking Union of Medical College, Tianjin, 300020, China

    Zhongchao Han

Authors
  1. Xiuli Wu
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  2. Yangqiu Li
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  3. Kanger Zhu
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  4. Zhen Wang
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  5. Shaohua Chen
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  6. Lijian Yang
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  7. Zhongchao Han
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Corresponding author

Correspondence to Yangqiu Li.

Additional information

Supported by a grant from National Scaling Height Program, China (No. 95-zhuan-10).

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Wu, X., Li, Y., Zhu, K. et al. Expression pattern of GATA-1, -2 and -3 genes in leukemic bone marrow microenvironment. Chin. -Ger. J. Clin. Oncol. 8, 541–545 (2009). https://doi.org/10.1007/s10330-009-0101-z

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  • DOI: https://doi.org/10.1007/s10330-009-0101-z

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