Abstract
Objective
The aim of this study was to study the inhibition effect of small interfering RNAs (siRNA) on gene expression in MG-63 cells, and to study the inhibitory effect on metastasis of MG-63.
Methods
A plasmid of a short hairpin RNA targeting acid phosphatase 1 (ACP1) was constructed and transfected into MG-63 cell line. ACP1 expression of MG-63 cells before and after transfection was detected by RT-PCR and Western blot. The capacity of adhesion, migration and invasion was examined by adhesion assay, migration assay and transwell assay.
Results
The recombinant plasmid pGenesil-1/ACP1-shRNA was successfully constructed. shRNA efficiently inhibited the expression of ACP1 by gene and protein level and suppressed cell migration. The adhesion decreased from 96.41 ± 8.83 to 43.38 ± 6.03 (P < 0.01), invasion ability from 56.5 ± 4.8 to 36.3 ± 6.1 (P < 0.05).
Conclusion
Down-regulating ACP1 by shRNA reduced the capacity of metastasis of MG-63 cell, which providing a novo-approach to biotherapy of cancer.
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Zhu, B., Chen, A. & Guo, F. The influence of down-regulation of ACP1 by RNAi on the metastasis capability of osteosarcoma cell line MG-63. Chin. -Ger. J. Clin. Oncol. 8, 481–484 (2009). https://doi.org/10.1007/s10330-009-0097-4
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DOI: https://doi.org/10.1007/s10330-009-0097-4