Abstract
Objective
To study the clinical significance of human mammaglobin (hMAM) mRNA in peripheral blood of patients with breast cancer.
Methods
The expression of hMAM mRNA was detected in peripheral blood from patients with breast cancer, breast benign lesions, healthy volunteers by nested reverse transcription polymerase chain reaction (nested-RT-PCR) method. The possible correlations of hMAM mRNA expression with clinico-pathological parameters and related molecular markers such as p53, PCNA and HER-2 were analyzed. The hMAM mRNA changed in peripheral blood after chemotherapy was observed.
Results
The positive rate of hMAM mRNA was 34.0% in peripheral blood of breast cancer, hMAM mRNA was not expressed in peripheral blood from other cancers, breast benign lesions or healthy volunteers. The expression of hMAM mRNA in peripheral blood was correlated with lymph nodes status and TNM stage. After 2–3 cycles adjuvant chemotherapy, In 13 of 17 cases, the positive hMAM mRNA turned to be negative, and there was statistical difference between pre-chemotherapy and post-chemotherapy.
Conclusion
hMAM mRNA has specific expression in breast cancer, the positive expression of hMAM mRNA in peripheral blood might predict hematogenous metastatic spreading of tumor cells, and hMAM mRNA may be potential biological marker for detecting breast cancer micrometastasis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Miksicek RJ, Myal Y, Watson PH, et al. Identification of a novel breast- and salivary gland-specific, mucin-like gene strongly expressed in normal and tumor human mammary epithelium. Cancer Res, 2002, 62: 2736–2740.
Han ZX, Zhang JC, Du XP. Detection of PIP mRNA level in peripheral blood of patients with breast cancer and its clinical significance. Chin J Cancer Prev Treat (Chinese), 2006, 13: 1635–1637.
Janku F, Kleibl Z, Novotny J, et al. Mammaglobin A, a novel marker of minimal residual disease in early stages breast cancer. Neoplasma, 2004, 51: 204–208.
Ghaffari SR, Sabokbar T, Tahmasebi S, et al. Combining mammaglobin and carcinoembryonic mRNA markers for early detection of micrometastases from breast cancers - a molecular study of 59 patients. Asian Pac J Cancer Prev, 2006, 7: 396–398.
Zehentner BK, Persing DH, Deme A, et al. Mammaglobin as a novel breast cancer biomarker: multigene reverse transcription-PCR assay and sandwich ELISA. Clin Chem, 2004, 50: 2069–2076.
Roncella S, Ferro P, Bacigalupo B, et al. Human mammaglobin mRNA is a reliable molecular marker for detecting occult breast cancer cells in peripheral blood. J Exp Clin Cancer Res, 2005, 24: 265–271.
Bernstein JL, Godbold JH, Raptis G, et al. Identification of mammaglobin as a novel serum marker for breast cancer. Clin Cancer Res, 2005, 11: 6528–6535.
Ntoulia M, Stathopoulou A, Ignatiadis M, et al. Detection of mammaglobin A-mRNA-positive circulating tumor cells in peripheral blood of patients with operable breast cancer with nested RT-PCR. Clin Biochem, 2006, 39: 879–887.
Ferrucci PF, Rabascio C, Gigli F, et al. A new comprehensive gene expression panel to study tumor micrometastasis in patients with high-risk breast cancer. Int J Oncol, 2007, 30: 955–962.
Yu ZY, Song LH, Zhuo PY, et al. The clinical significance of hMAM mRNA assay in peripheral blood of patients with breast carcinoma. Chin J Curr Adv Gen Surg (Chinese), 2004, 7: 235–237.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xie, X., Zhang, F., Zheng, Z. et al. The clinical significance of hMAM mRNA expression in peripheral blood of patients with breast cancer. Chin. -Ger. J. Clin. Oncol. 7, 709–712 (2008). https://doi.org/10.1007/s10330-008-0088-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10330-008-0088-x