Abstract
Objective
To investigate the anti-proliferative effect of histone deacetylases-1 (HDAC-1) knockdown in Hela cells.
Methods
The HDAC-1 protein was knockdowned using siRNA. The expression of HDAC-1 was detected by Western blotting. Apoptosis was assessed by flow cytometry. The inhibition of cell growth was assesses by MTT assay.
Results
HDAC-1 siRNA knockdowned the expression of HDAC-1 protein. HDAC siRNA inhibited the proliferation of Hela cells. HDAC-1 siRNA induced apoptosis.
Conclusion
HDAC-1 siRNA may inhibit the growth of Hela cells by inducing apoptosis.
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Li, N., Yu, S. The proliferation inhibition of Hela cells by histone deacetylases-1 siRNA. Chin. -Ger. J. Clin. Oncol. 7, 365–367 (2008). https://doi.org/10.1007/s10330-008-0034-y
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DOI: https://doi.org/10.1007/s10330-008-0034-y