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The study of special killing effect of CD3AK on anti-CEA-positive tumor enhanced by DC loaded with CEA-rV

  • Published:
The Chinese-German Journal of Clinical Oncology

Abstract

Objective

To survey the special killing activity of CD3AK on anti-CEA-positive tumor enhanced by umbilical cord blood dendritic cell (DC) loaded with CEA recombinant vaccinia virus (CEA-rV).

Methods

Freshly isolated umbilical blood mononuclear cells (UBMC) were cultivated for 3 h. Suspension cells and attached cells were used to induce CD3AK cells and DC separately. DC was loaded with CEA-rV on the 3rd day to prepare CEA-rV+DC. CD3AK cells were co-cultured with CEA-rV+DC on the 8th day, to prepare CEA-rV+DC+CD3AK. The killing activity of each effector’s cell, which included UBMC, CD3AK, DC+CD3AK and CEA-rV+DC+CD3AK, was measured respectively by MTT reduction assay.

Results

(1) 4 target cells were confirmed by CEA monoclonal antibody of rabbit anti-human. Lovo and A549 were really CEA positive cell lines, while Bel-7402 and K562 were CEA negative cell lines. (2) It was showed by flow-cytometry that the mature DC cultured at 10th day expressed MHC I, II molecules such as CD86, CD80, CD83 and CD40 highly, but CD123 lowly. The expression rates of CD86, CD80, CD83 and CD40 was 82.7%, 51.1%, 57.5% and 69.4%, respectively. The appearances and intra-cellular structures of DC were observed through light and electron microscope. The diameter of mature DC was 15–20 μm presented the irregular morphologic appearance, much prominences and pseudopodium. There were abundant mitochondria and endoplasmic reticulum in DC endochylema. (3) The rates of CD3, CD4, CD8 and CD28 in CD3AK cells group were 2 folds higher than that in UBMC group by FACS. It was said that the numbers of the mature T lymphocyte in CD3AK cells group were much greater than that in UBMC group. (4) The killing activities to 4 target cells of 3 effector’s cells, which included CEA-rV+DC+CD3AK, DC+CD3AK and CD3AK, were much greater than that of UBMC (P<0.01). Moreover, comparing with the killing activities of 4 effector’s: CEA-rV+DC+CD3AK group > DC+CD3AK group > CD3AK group > UBMC group. It showed that, cytokine, DC and CEA-rV could efficiently elevate the killing activity of UBMC on broad-spectrum tumor cells. (5) Comparing with the killing activities of CEA-rV+DC+CD3AK and CD3AK cells to CEA positive and negative cells, the killing activities of CEA-rV+DC+CD3AK to CEA positive tumor cells, Lovo and A549 cells (P<0.01) were remarkably better than that to CEA negative tumor cells BEL-7402 and K562 cells (P<0.05). It was said that the CEA-rV+DC could obviously enhance the killing activity of CD3AK on CEA positive tumor cells. Comparing with the killing activities of CEA-rV+DC+CD3AK and DC+CD3AK cells, the killing activity of CD3AK on CEA negative tumor cells was no statistical difference (P>0.05). However, the killing activity to CEA positive cells of CEA-rV+DC+CD3AK group was notably higher than that of DC+CD3AK group. Namely, CEA-rV could distinctly promote the special killing activity to CEA positive tumor cells of CD3AK, but could not do it to CEA negative tumor cells.

Conclusion

CEA-rV+DC could obviously enhance the special killing activity of CD3AK on CEA positive tumor cell lines, while the DC only couldn’t. The results indicated that the CEA-rV played an important role during the special killing activity of CD3AK cells to CEA positive tumor cells.

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References

  1. Hauck W, Stanners CP. Control of carcinoembryonic antigen gene family expression in a differentiating colon carcinoma cell line, Caco-2. Cancer Res, 1991, 51: 3526–3533.

    PubMed  CAS  Google Scholar 

  2. Luo CQ, Yang J, Lu FA, et al. The study of treatment and prevention on CEA-positive tumors by CEA-recombinant vaccinia virus. Acad J SUMS (Chinese), 2000, 21: 81–83.

    CAS  Google Scholar 

  3. Qi YC, Yang B, Zhao P. Actuality and prospect of dendritic cells in the tumor immunotherapy. China J Cancer Prev Treat (Chinese), 2002, 9: 434–436.

    Google Scholar 

  4. Timmerman JM, Czerwinski DK, Davis TA, et al. Idiotype-pulsed dendritic cell vaccination for B-cell lymphoma: clinical and immune responses in 35 patients. Blood, 2002, 99: 1517–1526.

    Article  PubMed  CAS  Google Scholar 

  5. Zhang J, Zhang JR, Wang SM, et al. CD3AK and LAK’s susceptibility and multi-drug resistance in KB and KBv200 cell line. Chin J Microbiol Immunol (Chinese), 2001, 21: 288–291.

    CAS  Google Scholar 

  6. Qi YC, Wang DZ, Yang B, et al. Preparation and bio-active assay of CD3AK cell from umbilical blood. Cancer Res Prev Treat (Chinese), 2004, 31: 381–383.

    Google Scholar 

  7. Yang J, Luo CQ, Lu FA, et al. Construction and production of CEA-recombinant vaccinia virus. Chin Biochem Mol Biol (Chinese), 1999, 15: 54–57.

    CAS  Google Scholar 

  8. Yang B, Qi YC, Lu MY. Proliferation of dendritic cells from human cord blood in vitro. Prac J Cancer (Chinese), 2004, 19: 117–120.

    Google Scholar 

  9. Mosmann T. Rapid colorimetric assay of cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983, 65: 55–63.

    Article  PubMed  CAS  Google Scholar 

  10. Hamada Y, Yamamura M, Hioki K, et al. Immunohistochemical study of carcinoembryonic antigen in patients with colorectal cancer. Cancer, 1985, 55: 136–141.

    Article  PubMed  CAS  Google Scholar 

  11. Ye F, Chen HZ, Xie X, et al. In vitro anti-tumor effect of cytotoxicity T lymphocytes activated by dendritic cells loaded with freeze-thaw ovarian carcinoma antigen. Chin J Microbiol Immunol (Chinese), 2004, 24: 964–967.

    CAS  Google Scholar 

  12. Xie YA, Luo XL, Liang AM, et al. Anti-tumor effect of CD3AK cells synergized with dendritic cells allergized by tumor antigen. Chin J Cancer Biother (Chinese), 2004, 11: 54–55.

    Google Scholar 

  13. Qi YC, Wang XS, Yang B, et al. Preventive and therapeutic effects of CEA recombinant vaccinia virus on CEA positive tumor. J Tumor Marker Oncol, 2004, 19: 409–413.

    Google Scholar 

  14. Wu J, Yang TC, Lai HW, et al. Induction of CEA-specific cytotoxic T lymphocytes by dendritic cells transfected with CEA-recombinant vaccinia virus. Cancer Res Prev Treat (Chinese), 2002, 29: 353–355.

    Google Scholar 

  15. Marshall JL, Gulley JL, Arlen PM, et al. Phase I study of sequential vaccination with Fowlpox-CEA (6D)-TRICOM alone and sequentially with vaccina-CEA (6D)-TRICOM, with and without granulocyte-macrophage colony-stimulating factor, in patients with carcinoembryonic antigen-expressing carcinomas. J Clinic Oncol, 2005, 23: 720–731.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Bioengineering, the Affiliated Tumor Hospital, Guangzhou Medical College, Guangzhou, 510095, China

    Yanchao Qi, Bo Yang, Minying Lu, Dongxiao Pan & Feng Liu

  2. Center of Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China

    Xinshuai Wang

Authors
  1. Yanchao Qi
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  2. Xinshuai Wang
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  3. Bo Yang
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  4. Minying Lu
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  5. Dongxiao Pan
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  6. Feng Liu
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Correspondence to Yanchao Qi.

Additional information

Supported by a grant from Science & Technique Department of Guangdong Province of China (No. 2002C30305).

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Qi, Y., Wang, X., Yang, B. et al. The study of special killing effect of CD3AK on anti-CEA-positive tumor enhanced by DC loaded with CEA-rV. Chinese German J Clin Oncol 5, 407–411 (2006). https://doi.org/10.1007/s10330-006-0489-7

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  • DOI: https://doi.org/10.1007/s10330-006-0489-7

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