No evidence for mitochondrial genetic variability in the largest population of critically endangered Tonkin snub-nosed monkeys in Vietnam
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The Tonkin snub-nosed monkey (Rhinopithecus avunculus) with a global population of <250 is listed as critically endangered. It is endemic to northeastern Vietnam and was feared extinct until its rediscovery in 1989. The largest single population of R. avunculus consists of 125–130 individuals in an area of forest called Khau Ca in Ha Giang Province. We used non-invasively collected fecal samples to establish the amount of genetic diversity in this population based on mitochondrial information. We amplified and sequenced a 467- to 650-bp section of the hypervariable region I (HVI) of the mitochondrial D-loop for 201 samples and reconstructed the full mitochondrial genomes for five samples based on metagenomic data. All 201 HVI sequences were identical and no variability was found in the five mitochondrial genomes. Our results highlight the immediate need for a comprehensive assessment of the genetic diversity of all populations of R. avunculus based on mitochondrial and nuclear markers. The latter need to be developed for this species.
KeywordsMitochondria D-loop Hypervariable region I Colobine Conservation
We thank everyone for their help, particularly the management board of the Tonkin Snub-nosed Monkey Species Habitat Conservation Area at Khau Ca; Mr Hoang Van Tue, Vietnam Administration of Forestry, Southern Institute of Ecology; Mr Dan Van Khoan, his family and his team; and the Evolutionary Biology Lab (National University of Singapore). We would also like to thank AIT Biotech for facilitating the NGS sequencing.
Compliance with Ethical Standards
We followed the Code of Best Practices for Field Primatology (2014) and the ethical guidelines of the University of Colorado Boulder and the National University of Singapore. Research permits were obtained and we followed the rules and regulations of the Forest Protection Department of Ha Giang, the Protected Area Management Board of Khau Ca, and relevant Vietnamese authorities (permit no. 2039/TCLN-BTTN).
This research was funded by Wildlife Reserves Singapore, the Mohamed bin Zayed Species Conservation Fund, the Beverly Sears Graduate Student Research Grant, the Scott Ferris Graduate Research Award, the Department of Anthropology Pre-Dissertation Grant, SEABIG (NUS) R-154-000-648-646 and R-154-000-648-733, and Dr Vilma D’Rozario.
Conflict of interest
The authors declare that they have no conflict of interest and gave final approval for publication.
- Ang A, Srivathsan A, Md-Zain BM, Ismail MRB, Meier R (2012) Low genetic variability in the recovering urban banded leaf monkey population of Singapore. Raffles B Zool 60:589–594Google Scholar
- Baillie JEM, Butcher ER (2012) Priceless or worthless? The world’s most threatened species. Zoological Society of London, UKGoogle Scholar
- Faircloth BC, Glenn TC (2014) Protocol: Preparation of an AMPure XP substitute (AKA Serapure). doi: 10.6079/J9MW2F26
- Garrison E, Marth G (2012) Haplotype-based variant detection from short-read sequencing. arXiv:1207.3907 [q-bio.GN]
- Le KQ (2002) Distribution and conservation of Tonkin snub-nosed monkey (Rhinopithecus avunculus) in Du Gia Nature Reserve, Ha Giang Province, Northeast Vietnam. Fauna and Flora International-Indochina ProgrammeGoogle Scholar
- Le KQ, Dong TH, Nadler T (2015) Tonkin snub-nosed monkey Rhinopithecus avunculus (Dollman 1912). In: Schwitzer C, Mittermeier RA, Rylands AB, Chiozza F, Williamson EA, Wallis J, Cotton A (eds) Primates in peril: the world’s 25 most endangered primates 2014–2016. IUCN SSC PSG, IPS, CI, and Bristol Zoological Society, Arlington, pp 58–59Google Scholar
- Mittermeier RA, Cheney DL (1987) Conservation of primates and their habitats. In: Smuts BB, Cheney DL, Seyfarth RM, Wrangham RW, Strushsaker TT (eds) Primate societies. University of Chicago Press, Chicago, pp 477–490Google Scholar
- Nadler T (2014) Habitat disturbance and loss, and the primates of Vietnam. In: Nadler T, Brockman D (eds) Primates of Vietnam. Endangered Primate Rescue Center, Cuc Phuong National Park, Vietnam, pp 55–60Google Scholar
- Ratajszczak R, Can Dang Ngoc, Nhat Pham (1992) A survey for Tonkin snub-nosed monkey (Rhinopithecus avunculus) in the North Vietnam. FFI Preservation Society and WWF International, London and GlandGoogle Scholar
- Roos C, Thanh Vu Ngoc, Walter L, Nadler T (2007) Molecular systematics of Indochinese primates. Vietnam J Primatol 1:41–53Google Scholar
- Roos C, Zinner D, Kubatko LS, Schwarz C, Yang M, Meyer D, Nash SD, Xing J, Batzer MA, Brameier M, Leendertz FH, Ziegler T, Perwitasari-Farajallah D, Nadler T, Walter L, Osterholz M (2011) Nuclear versus mitochondrial DNA: evidence for hybridization in colobine monkeys. BMC Evol Biol 11:77CrossRefPubMedPubMedCentralGoogle Scholar
- Yang M, Yang Y, Cui D, Fickenscher G, Zinner D, Roos C, Brameier M (2012) Population genetic structure of Guizhou snub-nosed monkeys (Rhinopithecus brelichi) as inferred from mitochondrial control region sequences, and comparison with R. roxellana and R. bieti. Am J Phys Anthropol 147:1–10CrossRefPubMedGoogle Scholar