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Seroprevalence of Japanese encephalitis virus infection in captive Japanese macaques (Macaca fuscata)

Primates volume 55, pages 441–445 (2014)Cite this article

Abstract

Japanese encephalitis virus (JEV), which is transmitted by mosquitoes, infects many animal species and causes serious acute encephalitis in humans and horses. In this study, a serosurvey of JEV in Japanese macaques (Macaca fuscata) reared in Aichi Prefecture was conducted using purified JEV as an antigen for ELISA. The results revealed that 146 of 332 monkeys (44 %) were seropositive for JEV. In addition, 35 of 131 monkeys (27 %) born in the facility were seropositive, and the annual infection rate in the facility was estimated as 13 %. Our results provide evidence of the frequent exposure of many Japanese macaques to JEV, suggesting that there is a risk of JEV transmission to humans by mosquitoes.

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References

  • Abe M, Okada K, Hayashida K, Matsuo F, Shiosaki K, Miyazaki C, Ueda K, Kino Y (2007) Duration of neutralizing antibody titer after Japanese encephalitis vaccination. Microbiol Immunol 51:609–616

    CAS  PubMed  Article  Google Scholar 

  • Arai S, Matsunaga Y, Takasaki T, Tanaka-Taya K, Taniguchi K, Okabe N, Kurane I, Vaccine Preventable Diseases Surveillance Program of Japan (2008) Japanese encephalitis: surveillance and elimination effort in Japan from 1982 to 2004. Jpn J Infect Dis 61:333–338

    PubMed  Google Scholar 

  • Erlanger TE, Weiss S, Keiser J, Utzinger J, Wiedenmayer K (2009) Past, present, and future of Japanese encephalitis. Emerg Infect Dis 15:1–7

    PubMed Central  PubMed  Article  Google Scholar 

  • Hamano M, Lim CK, Takagi H, Sawabe K, Kuwayama M, Kishi N, Kurane I, Takasaki T (2007) Detection of antibodies to Japanese encephalitis virus in the wild boars in Hiroshima prefecture, Japan. Epidemiol Infect 135:974–977

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Inoshima Y, Shimizu S, Minamoto N, Hirai K, Sentsui H (1999) Use of protein AG in an enzyme-linked immunosorbent assay for screening for antibodies against parapoxvirus in wild animals in Japan. Clin Diagn Lab Immunol 6:388–391

    CAS  PubMed Central  PubMed  Google Scholar 

  • Inoue S, Morita K, Matias RR, Tuplano JV, Resuello RR, Candelario JR, Cruz DJ, Mapua CA, Hasebe F, Igarashi A, Natividad FF (2003) Distribution of three arbovirus antibodies among monkeys (Macaca fascicularis) in the Philippines. J Med Primatol 32:89–94

    CAS  PubMed  Article  Google Scholar 

  • Konishi E, Kitai Y (2009) Detection by ELISA of antibodies to Japanese encephalitis virus nonstructural 1 protein induced in subclinically infected humans. Vaccine 27:7053–7058

    CAS  PubMed  Article  Google Scholar 

  • Konishi E, Suzuki T (2002) Ratios of subclinical to clinical Japanese encephalitis (JE) virus infections in vaccinated populations: evaluation of an inactivated JE vaccine by comparing the ratios with those in unvaccinated populations. Vaccine 21:98–107

    PubMed  Article  Google Scholar 

  • Mackenzie JS, Chua KB, Daniels PW, Eaton BT, Field HE, Hall RA, Halpin K, Johansen CA, Kirkland PD, Lam SK, McMinn P, Nisbet DJ, Paru R, Pyke AT, Ritchie SA, Siba P, Smith DW, Smith GA, van den Hurk AF, Wang LF, Williams DT (2001) Emerging viral diseases of Southeast Asia and the Western Pacific. Emerg Infect Dis 7:497–504

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Marassa AM, Paula MB, Gomes AC (2009) Biotin-avidin sandwich ELISA with specific human isotypes IgG1 and IgG4 for culicidae mosquito blood meal identification from an epizootic yellow fever area in Brazil. J Venom Anim Toxins Incl Trop Dis 15:696–706

    Google Scholar 

  • Matsunaga T, Shoda M, Konishi E (2008) Japanese encephalitis viral infection remains common in Japan. Pediatr Infect Dis J 27:769–770

    PubMed  Article  Google Scholar 

  • Nakgoi K, Nitatpattana N, Wajjwalku W, Pongsopawijit P, Kaewchot S, Yoksan S, Siripolwat V, Souris M, Gonzalez JP (2014) Dengue, Japanese encephalitis and Chikungunya virus antibody prevalence among captive monkey (Macaca nemestrina) colonies of Northern Thailand. Am J Primatol 76:97–102

    PubMed  Article  Google Scholar 

  • Nerome R, Tajima S, Takasaki T, Yoshida T, Kotaki A, Lim CK, Ito M, Sugiyama A, Yamauchi A, Yano T, Kameyama T, Morishita I, Kuwayama M, Ogawa T, Sahara K, Ikegaya A, Kanda M, Hosoya Y, Itokazu K, Onishi H, Chiya S, Yoshida Y, Tabei Y, Katsuki K, Tabata K, Harada S, Kurane I (2007) Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004. J Gen Virol 88:2762–2768

    CAS  PubMed  Article  Google Scholar 

  • Nidaira M, Taira K, Itokazu K, Kudaka J, Nakamura M, Ohno A, Takasaki T (2007) Survey of the antibody against japanese encephalitis virus in Ryukyu wild boars (Sus scrofa riukiuanus) in Okinawa, Japan. Jpn J Infect Dis 60:309–311

    PubMed  Google Scholar 

  • Ohno Y, Sato H, Suzuki K, Yokoyama M, Uni S, Shibasaki T, Sashika M, Inokuma H, Kai K, Maeda K (2009) Detection of antibodies against Japanese encephalitis virus in raccoons, raccoon dogs and wild boars in Japan. J Vet Med Sci 71:1035–1039

    PubMed  Article  Google Scholar 

  • Oya A, Kurane I (2007) Japanese encephalitis for a reference to international travelers. J Travel Med 14:259–268

    PubMed  Article  Google Scholar 

  • Peiris JS, Dittus WP, Ratnayake CB (1993) Seroepidemiology of dengue and other arboviruses in a natural population of toque macaques (Macaca sinica) at Polonnaruwa, Sri Lanka. J Med Primatol 22:240–245

    CAS  PubMed  Google Scholar 

  • Shimoda H, Ohno Y, Mochizuki M, Iwata H, Okuda M, Maeda K (2010) Dogs as sentinels for human infection with Japanese encephalitis virus. Emerg Infect Dis 16:1137–1139

    PubMed Central  PubMed  Article  Google Scholar 

  • Shimoda H, Tamaru S, Morimoto M, Hayashi T, Shimojima M, Maeda K (2011) Experimental infection of Japanese encephalitis virus in dogs. J Vet Med Sci 73:1241–1242

    PubMed  Article  Google Scholar 

  • Shimoda H, Inthong N, Noguchi K, Terada Y, Nagao Y, Shimojima M, Takasaki T, Rerkamnuaychoke W, Maeda K (2013) Development and application of an indirect enzyme-linked immunosorbent assay for serological survey of Japanese encephalitis virus infection in dogs. J Virol Methods 187:85–89

    CAS  PubMed  Article  Google Scholar 

  • Yamane Y (1974) Natural virus infection in green and cynomolgus monkeys. Kitasato Arch Exp Med 47:15–66

    CAS  PubMed  Google Scholar 

  • Yuwono J, Suharyono W, Koiman I, Tsuchiya Y, Tagaya I (1984) Seroepidemiological survey on dengue and Japanese encephalitis virus infections in Asian monkeys. Southeast Asian J Trop Med Public Health 15:194–200

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by a Grant-in-Aid for a Japan Society for the Promotion of Science Fellowship and a grant from the Ministry of Health, Labour, and Welfare of Japan (H20-Shinko-Ippan-003).

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Affiliations

  1. Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, Yamaguchi, 753-8515, Japan

    Hiroshi Shimoda, Keita Noguchi, Yutaka Terada, Ryusei Kuwata & Ken Maeda

  2. Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, 41-2 Kanrin, Inuyama, Aichi, 484-8506, Japan

    Akatsuki Saito & Hirofumi Akari

  3. Division 2, Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjyuku, Tokyo, 162-8640, Japan

    Tomohiko Takasaki

Authors
  1. Hiroshi Shimoda
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  2. Akatsuki Saito
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  3. Keita Noguchi
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  4. Yutaka Terada
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  5. Ryusei Kuwata
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  6. Hirofumi Akari
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  7. Tomohiko Takasaki
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  8. Ken Maeda
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Corresponding author

Correspondence to Ken Maeda.

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Shimoda, H., Saito, A., Noguchi, K. et al. Seroprevalence of Japanese encephalitis virus infection in captive Japanese macaques (Macaca fuscata). Primates 55, 441–445 (2014). https://doi.org/10.1007/s10329-014-0421-7

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  • DOI: https://doi.org/10.1007/s10329-014-0421-7

Keywords

  • ELISA
  • Japanese encephalitis virus
  • Macaca fuscata
  • Monkeys