Skip to main content

Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis)

Primates volume 48, pages 140–146 (2007)Cite this article

Abstract

To develop a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis), 148 microsatellite markers were selected from the human genome database. The polymorphisms and inheritance of PCR products were determined by screening twenty unrelated monkeys and by analysis of three families, respectively. As a result, 106 primers (72%) gave PCR products of the size expected for humans and rhesus monkeys. Among these products, polymorphism and single-gene inheritance in cynomolgus monkeys was observed for 66 markers (62%). The average number of alleles at the 66 polymorphic loci was 5.86 (range 2–10), and average heterozygosity was 0.63 (range 0.10–0.88). This is the first report of microsatellite markers for cynomolgus monkeys. Chromosomal mapping of these markers is now in progress.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Ami Y, Suzaki Y, Goto N (1993) Endometriosis in cynomolgus monkeys retired from breeding. J Vet Med Sci 55:7–11

    CAS  Article  PubMed  Google Scholar 

  • Andrade MC, Penedo MC, Ward T, Silva VF, Bertolini LR, Roberts JA, Leite JP, Cabello PH (2004) Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta). Primates 45:183–186

    Article  PubMed  Google Scholar 

  • Domingo-Roura X, Lopez-Giraldez T, Shinohara M, Takenaka O (1997) Hypervariable microsatellite loci in the Japanese macaque (Macaca fuscata) conserved in related species. Am J Primatol 43:357–360

    CAS  Article  PubMed  Google Scholar 

  • Foissac A, Salhi M, Cambon-Thomsen A (2000) Microsatellites in the HLA region: 1999 update. Tissue Antigens 55:477–509

    CAS  Article  PubMed  Google Scholar 

  • Hadfield RM, Pullen JG, Davies KF, Wolfensohn SE, Kemnitz JW, Weeks DE, Bennett ST, Kennedy SH (2001) Toward developing a genome-wide microsatellite marker set for linkage analysis in the rhesus monkey (Macaca mulatta): identification of 76 polymorphic markers. Am J Primatol 54:223–231

    CAS  Article  PubMed  Google Scholar 

  • Havill LM, Mahaney MC, Cox LA, Morin PA, Joslyn G, Rogers J (2005) A quantitative trait locus for normal variation in forearm bone mineral density in pedigreed baboons maps to the ortholog of human chromosome 11q. J Clin Endocrinol Metab 90:3638–3645

    CAS  Article  PubMed  Google Scholar 

  • Lukas D, Bradley BJ, Nsubuga AM, Doran-Sheehy D, Robbins MM, Vigilant L (2004) Major histocompatibility complex and microsatellite variation in two populations of wild gorillas. Mol Ecol 13:3389–3402

    CAS  Article  PubMed  Google Scholar 

  • Martin MP, Harding A, Chadwick R, Kronick M, Cullen M, Lin L, Mignot E, Carrington M (1998) Characterization of 12 microsatellite loci of the human MHC in a panel of reference cell lines. Immunogenetics 47:131–138

    CAS  Article  PubMed  Google Scholar 

  • Morin PA, Moore JJ, Chakraborty R, Jin L, Goodall J, Woodruff DS (1994a) Kin selection, social structure, gene flow, and the evolution of chimpanzees. Science 265:1193–1201

    CAS  Article  Google Scholar 

  • Morin PA, Wallis J, Moore JJ, Woodruff DS (1994b) Paternity exclusion in a community of wild chimpanzees using hypervariable simple sequence repeats. Mol Ecol 3:469–477

    CAS  Article  Google Scholar 

  • Nei M, Roychoudhury AK (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76:379–390

    CAS  PubMed  PubMed Central  Google Scholar 

  • Rogers J, Mahaney MC, Witte SM, Nair S, Newman D, Wedel S, Rodriguez LA, Rice KS, Slifer SH, Perelygin A, Slifer M, Palladino-Negro P, Newman T, Chambers K, Joslyn G, Parry P, Morin PA (2000) A genetic linkage map of the baboon (Papio hamadryas) genome based on human microsatellite polymorphisms. Genomics 67:237–247

    CAS  Article  PubMed  Google Scholar 

  • Rogers J, Garcia R, Shelledy W, Kaplan J, Arya A, Johnson Z, Bergstrom M, Novakowski L, Nair P, Vinson A, Newman D, Heckman G, Cameron J (2006) An initial genetic linkage map of the rhesus macaque (Macaca mulatta) genome using human microsatellite loci. Genomics 87:30–38

    CAS  Article  PubMed  Google Scholar 

  • Smith DG, Kanthaswamy S, Viray J, Cody L (2000) Additional highly polymorphic microsatellite (STR) loci for estimating kinship in rhesus monkeys (Macaca mulatta). Am J Primatol 50:1–7

    CAS  Article  PubMed  Google Scholar 

  • Suzuki MT, Terao K, Yoshikawa Y (2003) Familial early onset macular degeneration in cynomolgus monkeys (Macaca fascicularis). Primates 44:291–294

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We are grateful to Dr Duncan A. Vaughan (The National Institute of Agrobiological Sciences, Japan) for critical reading and editing of the manuscript. This study was supported by a grant (H15-genome-004) from the Ministry of Health, Labor, and Welfare, Japan.

Author information

Affiliations

  1. Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, Tskukuba, Ibaraki, 305-0843, Japan

    Toshihiko Kikuchi, Masayuki Hara & Keiji Terao

Authors
  1. Toshihiko Kikuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masayuki Hara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Keiji Terao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Keiji Terao.

About this article

Cite this article

Kikuchi, T., Hara, M. & Terao, K. Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis). Primates 48, 140–146 (2007). https://doi.org/10.1007/s10329-006-0008-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10329-006-0008-z

Keywords

  • Microsatellite
  • Cynomolgus monkeys
  • Genome-wide screening