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The first report of a rot on Welsh onion (Allium fistulosum L.) caused by Burkholderia cepacia complex bacteria in Akita Prefecture, Japan

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Abstract

In July 2021 in Akita Prefecture, a light-brown water-soaked lesions appeared on Welsh onion starting where the green leaves diverged, then spreading outward, and extending as water-soaked stripes to the white pseudostem. Bacteria isolated from the lesions were considered to belong to the Burkholderia cepacia complex (Bcc). Based on their pathogenicity on Welsh onion, bacteriological properties and genetic characteristics, the isolates were identified as B. cepacia, B. cenocepacia and B. pyrrocinia. This is the first report of bacterial rot of Welsh onion caused by Bcc bacteria in Japan.

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References

  • Azegami K, Fukuda M, Inoue Y (2013) Occurrence of bacterial rot of Welsh onion caused by Pseudomonas viridiflava. (Abstract in Japanese). Jpn J Phytopathol 79:34

    Google Scholar 

  • e-Stat (Portal site for Japanese Government Statistics) (2020) Statistical survey of crops (Accessed 2023 Mar 22). https://www.e-stat.go.jp/dbview?sid=0001922209

  • Iitomi A, Umekawa M, Taguchi T, Sasaki T (1983) The rot symptoms of Welsh onion caused by genus Pseudomonas bacteria (1). (Abstract in Japanese). Jpn J Phytopathol 49:415

    Google Scholar 

  • Inden H, Asahira T (1990) Japanese bunching onion (Allium fistulosum L.). In: Brewster JL, Rabinowitch HD (eds) Onions and allied crops, vol III Biochemistry, food sciences, and minor crops. CRC Press, Boca Raton, FL

    Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kusumoto S, Aeny TN, Mujimu S, Ginting C, Tsuge T, Tsuyumu S, Takikawa Y (2004) Occurrence of blood disease of banana in Sumatra, Indonesia. J Gen Plant Pathol 70:45–49

    Article  Google Scholar 

  • Maeda Y, Shinohara H, Kiba A, Ohnishi K, Furuya N, Kawamura Y, Ezaki T, Vandamme P, Tsushima S, Hikichi Y (2006) Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences. Int J Syst Evol Microbiol 56:1031–1038

    Article  CAS  PubMed  Google Scholar 

  • Mahenthiralingam E, Bischof J, Byrne SK, Radomski C, Davies JE, Av-Gay Y, Vandamme P (2000) DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol 38:3165–3173

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Misawa T, Yasuoka S (2012) The life cycle of Stemphylium vesicarium, the causal agent of Welsh onion leaf blight. J Gen Plant Pathol 78:18–29

    Article  Google Scholar 

  • Nishiyama K, Ezuka A (1977) Pseudomonas coronafaciens var. Atropurpurea obtained from diseased leaves of ryegrasses (in japanese). Jpn J Phytopathol 43:426–431

    Article  Google Scholar 

  • Sawada H, Fujikawa T, Nishiwaki Y, Horita H (2020) Pseudomonas kitaguniensis sp. nov., a pathogen causing bacterial rot of Welsh onion. (Abstract in Japanese). Jpn J Phytopathol 86:232

    Google Scholar 

  • Sotokawa N, Takikawa Y (2004) Occurrence of bacterial rot of onion bulbs caused by Burkholderia cepacia in Japan. J Gen Plant Pathol 70:348–352

    Article  Google Scholar 

  • Suzuki A, Togawa M, Ohta K, Takikawa Y (2003) Occurrence of white top of pea caused by a new strain of Pseudomonas syringae pv. Pisi. Plant Dis 87:1404–1410

    Article  CAS  PubMed  Google Scholar 

  • Takahashi F, Ogiso H, Fujinaga M, Ishiyama Y, Inoue Y, Shirakawa T, Takikawa Y (2013) First report of bacterial blight of crucifers caused by Pseudomonas cannabina pv. Alisalensis in Japan. J Gen Plant Pathol 79:260–269

    Article  CAS  Google Scholar 

  • Takikawa Y, Serizawa S, Ichikawa T, Tsuyumu S, Goto M (1989) Pseudomonas syringae pv. Actinidiae nov.: the causal bacterium of canker of kiwifruit in Japan. Ann Phytopathol Soc Jpn 55:437–444

    Article  Google Scholar 

  • Tsuji M, Kadota I (2020) Identification and phylogenetic analysis of Burkholderia cepacia complex bacteria isolated from rot of onion bulbs in Tohoku region of Japan. J Gen Plant Pathol 86:376–386

    Article  CAS  Google Scholar 

  • Tsuji M, Takikawa Y (2018) Pseudomonas syringae pv. Alliifistulosi pv. nov., the causal agent of bacterial leaf spot of onions. J Gen Plant Pathol 84:343–358

    Article  CAS  Google Scholar 

  • Tsuji M, Ohta K, Tanaka K, Takikawa Y (2017) Comparison among japanese isolates of Pseudomonas savastanoi pv. Savastanoi, causal agent of olive knot disease. J Gen Plant Pathol 83:152–161

    Article  CAS  Google Scholar 

  • Umekawa M, Iitomi A, Sasaki T (1983) The rot symptoms of Welsh onion caused by genus Pseudomonas bacteria (2). (Abstract in Japanese). Jpn J Phytopathol 49:415

    Google Scholar 

  • Vandamme P, Holmes B, Vancanneyt M, Coenye T, Hoste B, Coopman R, Revets H, Lauwers S, Gillis M, Kersters K, Govan JRW (1997) Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol 47:1188–1200

    Article  CAS  PubMed  Google Scholar 

  • Yabuuchi E, Kosako Y, Oyaizu H, Yano I, Hotta H, Hashimoto Y, Ezaki T, Arakawa M (1992) Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275

    Article  CAS  PubMed  Google Scholar 

  • Yamamoto S, Bouvet PJM, Harayama S (1999) Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization. Int J Syst Bacteriol 49:87–95

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We sincerely thank Dr. Yuichi Takikawa (Laboratory of Plant Pathology, Graduate School of Science and Technology, Shizuoka University), Dr. Yasuhiro Inoue (National Agriculture and Food Research Organization, Plant Protection Division) and Mr. Kazuhiko Sakai (Saitama Prefectural Agriculture and Forestry Research Center) for their kind and precious advice on pathogenic bacterial isolates from Welsh onion.

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Authors and Affiliations

  1. National Agriculture and Food Research Organization, Tohoku Agricultural Research Center, 4 Akahira Shimo-kuriyagawa, Morioka, Iwate, 020-0198, Japan

    Mizue Tsuji, Takaaki Saito & Motomu Honjou

  2. Department of Agro-environmental Research, Akita Pref. Agricultural Experiment Station, 34-1 Yuuwa Aikawa Genpachizawa, Akita, Akita, 010-1231, Japan

    Takaaki Saito

  3. Department of Vegetable and Flower Research, Akita Pref. Agricultural Experiment Station, 34-1 Yuuwa Aikawa Genpachizawa, Akita, Akita, 010-1231, Japan

    Motomu Honjou

Authors
  1. Mizue Tsuji
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  2. Takaaki Saito
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  3. Motomu Honjou
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Correspondence to Mizue Tsuji.

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Tsuji, M., Saito, T. & Honjou, M. The first report of a rot on Welsh onion (Allium fistulosum L.) caused by Burkholderia cepacia complex bacteria in Akita Prefecture, Japan. J Gen Plant Pathol 89, 238–243 (2023). https://doi.org/10.1007/s10327-023-01128-2

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  • DOI: https://doi.org/10.1007/s10327-023-01128-2

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