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Taxonomical identification of Chinese cabbage yellows inhibitory fungus isolated from disease suppressive soil

  • Fungal Diseases
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Abstract

A Chinese cabbage yellows-suppressing fungal strain (S69) isolated from a suppressive field soil to the disease was identified to be Pseudothielavia terricola, based on phylogenetic analyses and morphological observations. Phylogenetic studies were completed using individual and combined data sequences of ITS-LSU, rpb2, and tub2, and indicated that the identified fungal strain belonged to a monophyletic clade that also included P. arxii and P. terricola. Morphological observations revealed that the fungal ascospores had apical germ pores, a distinctive characteristic of P. terricola but not of P. arxii. Additional observed morphological characteristics of S69 were also similar to P. terricola.

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Acknowledgements

This work was supported by a project commissioned under the grant entitled “Development of field checkup system for soil-borne disease management using artificial intelligence,” funded by the Ministry of Agriculture, Forestry and Fisheries, Japan.

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Authors and Affiliations

  1. National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Masako Tsujimoto Noguchi, Haruka Nagase, Norihito Yamauchi & Shigenobu Yoshida

  2. Toyama Prefectural Agricultural, Forestry and Fisheries Research Center, 1124-1 Yoshioka, Toyama, Toyama, 939-8153, Japan

    Genki Mimuro

  3. Gunma Agricultural Technology Center, Eastern Regional Research Center, 1132-2 Togoucho, Tatebayashi, Gunma, 374-0006, Japan

    Hiroshi Sakai

  4. Gunma Prefectural Institute of Agricultural and Forestry, 1005 Nishiakiya, Misatomachi, Takasaki, Gunma, 370-3105, Japan

    Akiko Furusawa

  5. Gunma Prefectural Office, 493 Obokatacho, Isesaki, Gunma, 379-2224, Japan

    Shizue Miki

Authors
  1. Masako Tsujimoto Noguchi
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  2. Haruka Nagase
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  3. Norihito Yamauchi
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  4. Genki Mimuro
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  5. Hiroshi Sakai
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  6. Akiko Furusawa
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  7. Shizue Miki
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  8. Shigenobu Yoshida
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Correspondence to Masako Tsujimoto Noguchi.

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Accession Nos. LC565012, LC612778, and LC612779.

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Fig. S1

Phylogenetic tree of strain S69 and the related species of Chaetomiaceae based on the maximum likelihood (ML) analysis of partial ITS-LSU region sequences. Bootstrap values (1000 replicates) from ML analysis are shown near the nodes. Strain S69 is indicated in bold. The bar scale represents the expected number of changes per site. The strain numbers with a letter T mean type strains. Ex-epitype strain and ex-neotype strains are marked eT and Neo T, respectively. Microthecium fimicola was used as an outgroup species. Fig. S2 Phylogenetic tree of strain S69 and the related species of Chaetomiaceae based on the maximum likelihood (ML) analysis of the partial rpb2 gene sequences. Bootstrap values (1000 replicates) from ML analysis are shown near nodes. The scale bar represents the expected number of changes per site. The strain numbers with a letter T mean type strains. Ex-epitype strain and ex-neotype strain are marked eT and Neo T, respectively. Microthecium fimicola was used as an outgroup species. Fig. S3 Phylogenetic tree for the strain S69 and the related species of Chaetomiaceae based on the maximum likelihood (ML) analysis of the partial tub2 gene sequences. Bootstrap values (1000 replicates) from ML analysis are shown near the nodes. The bar scale represents the expected number of changes per site. The strain numbers with a letter T mean type strains. Ex-epitype strain and ex-neotype strains are marked eT and Neo T, respectively. Microthecium fimicola was used as an outgroup species (DOCX 442 KB)

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Noguchi, M.T., Nagase, H., Yamauchi, N. et al. Taxonomical identification of Chinese cabbage yellows inhibitory fungus isolated from disease suppressive soil. J Gen Plant Pathol 88, 239–245 (2022). https://doi.org/10.1007/s10327-022-01058-5

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