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Mitochondrial dynamics in Pyricularia oryzae during conidiation monitored by fluorescent microscopy


Pyricularia oryzae, which causes blast disease in rice, is a serious fungal pathogen of many cereal crops. Mitochondria have been a promising target to develop antifungal agents. We observed the movement and morphology of mitochondria during conidiation using GFP fused with citrate synthase in Ina 86-137 strain with a slide culture technique. During early conidiation, mitochondria were tubular and later changed to dot shape. This event is also synchronized with septum formation and discontinued movement of mitochondria from the conidiophore. This is the first description of morphology of the conidial mitochondria of P. oryzae from protuberance appearance to young conidia.

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  1. Asibi AE, Chai Q, Coulter JA (2019) Rice blast: a disease with implications for global food security. Agronomy 9:451

    CAS  Article  Google Scholar 

  2. Castroagudín VL, Ceresini PC, de Oliveira SC, Reges JTA, Maciel JLN, Bonato ALV, Dorigan AF, McDonald BA (2015) Resistance to QoI fungicides is widespread in Brazilian populations of the wheat blast pathogen Magnaporthe oryzae. Phytopathology 105:284–294

    Article  Google Scholar 

  3. Delettre C, Lenaers G, Griffoin JM, Gigarel N, Lorenzo C, Belenguer P, Pelloquin L, Grosgeorge J, Turc-Carel C, Perret E, Astarie-Dequeker C, Lasquellec L, Arnaud B, Ducommun B, Kaplan J, Hamel CP (2000) Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy. Nat Genet 26:207–210

    CAS  Article  Google Scholar 

  4. Hayashi K, Suzuki F, Hayano-Saito Y (2017) Multiplex PCR assay for simultaneous detection of MBI-D and QoI resistance in rice blast fungus. J Gen Plant Pathol 83:304–309

    CAS  Article  Google Scholar 

  5. Ishii H (2011) Fungicide resistance in rice. In: Dehne HW, Deising HB, Gisi U, Kuck KH, Russell PE, Lyr H (eds) Modern fungicides and antifungal compounds VI. DPG Selbstverlag, Braunschweig, pp 35–40

    Google Scholar 

  6. Ishii H (2015) Rice pathogens in Japan. In: Ishii H, Hollomon DW (eds) Fungicide resistance in plant pathogens. Springer, Japan, pp 341–354

    Chapter  Google Scholar 

  7. Khan IA, Ning G, Liu X, Feng X, Lin F, Lu J (2015) Mitochondrial fission protein MoFis1 mediates conidiation and is required for full virulence of the rice blast fungus Magnaporthe oryzae. Microbiol Res 178:51–58

    CAS  Article  Google Scholar 

  8. Li D, Calderone R (2017) Exploiting mitochondria as targets for the development of new antifungals. Virulence 8:159–168

    CAS  Article  Google Scholar 

  9. Madeo F, Fröhlich E, Ligr M, Grey M, Sigris SJ, Wolf DH, Fröhlich KU (1999) Oxygen stress: a regulator of apoptosis in yeast. J Cell Biol 145:757–767

    CAS  Article  Google Scholar 

  10. Mendoza H, Perlin MH, Schirawski J (2020) Mitochondrial inheritance in phytopathogenic fungi—everything is known, or is it? Int J Mol Sci 21:3883

    CAS  Article  Google Scholar 

  11. Miyagawa N, Fuji M, Kawabata Y (2013) Occurrence of orysastrobin-resistant isolates of rice blast fungus (Abstract in Japanese). Jpn J Phytopathol 79:197

    Google Scholar 

  12. Nalley L, Tsiboe F, Durand-Morat A, Shew A, Thoma G (2016) Economic and environmental impact of rice blast pathogen (Magnaporthe oryzae) alleviation in the United States. PLoS ONE 11:e0167295

    Article  Google Scholar 

  13. Nunnari J, Suomalainen A (2012) Mitochondria: in sickness and in health. Cell 148:1145–1159

    CAS  Article  Google Scholar 

  14. Osman C, Noriega TR, Okreglak V, Fung JC, Walter P (2015) Integrity of the yeast mitochondrial genome, but not its distribution and inheritance, relies on mitochondrial fission and fusion. Proc Natl Acad Sci USA 112:E947–E956

    CAS  Article  Google Scholar 

  15. Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675

    CAS  Article  Google Scholar 

  16. Syib’li MA, Kodama A, Abe A, Sone T (2020) Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA). J Gen Plant Pathol 86:250–256

    Article  Google Scholar 

  17. Wilson RA, Talbot NJ (2009) Under pressure: investigating the biology of plant infection by Magnaporthe oryzae. Nat Rev Microbiology 7:185–195

    CAS  Article  Google Scholar 

  18. Zemirli N, Morel E, Molino D (2018) Mitochondrial dynamics in basal and stressful conditions. Int J Mol Sci 19:E564

    Article  Google Scholar 

  19. Zeng XQ, Chen GQ, Liu XH, Dong B, Shi HB, Lu JP, Lin F (2014) Crosstalk between SNF1 pathway and the peroxisome-mediated lipid metabolism in Magnaporthe oryzae. PLoS ONE 9:e103124

    Article  Google Scholar 

  20. Zhong K, Li X, Le X, Kong X, Zhang H, Zheng X, Wang P, Zhang Z (2016) MoDnm1 dynamin mediating peroxisomal and mitochondrial fission in complex with MoFis1 and MoMdv1 is important for development of functional appressorium in Magnaporthe oryzae. PLoS Pathog 12:e1005823

    Article  Google Scholar 

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The authors thank Dr. Izumi Chuma (Obihiro University for Agriculture and Veterinary Medicine) for instruction on the slide culture technique. This work was supported by JSPS KAKENHI Grant Number JP18K05640.

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Correspondence to Teruo Sone.

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Balagalla, D.N., Abe, A. & Sone, T. Mitochondrial dynamics in Pyricularia oryzae during conidiation monitored by fluorescent microscopy. J Gen Plant Pathol (2021).

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  • Pyricularia oryzae
  • Mitochondria
  • Citrate synthase (Cit A)
  • Conidiation