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Suppressive effect of secondary metabolites from Streptomyces plumbeus isolate F31D against Fusarium oxysporum f. sp. lycopersici, the causal agent of Fusarium wilt of tomato

  • Fungal Diseases
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Abstract

Fusarium wilt of tomato, a disease caused by the soilborne fungus Fusarium oxysporum f. sp. lycopersici, causes major losses to tomato production. Chemical fungicides and resistant cultivars are the main control strategies, but new races of the pathogen appear to be able to overcome resistance genes in currently grown cultivars. Therefore, there is a need to identify natural antifungal compounds and develop new agents to control F. oxysporum f. sp. lycopersici. The culture filtrate (F31D-CF) from isolate F31D, a bacterial isolate collected from paddy field soil in Shimane Prefecture in 2019, significantly inhibited in vitro conidial germination of F. oxysporum f. sp. lycopersici, indicating a fungicidal effect against this pathogen. The amount of the inhibitory compound in F31D-CF increased from 1 to 3 days of incubation. F31D-CF significantly suppressed disease development in tomato plants. Sequence analysis of the 16S rDNA region of the isolate revealed 99% similarity with the type strain of Streptomyces plumbeus. Thin layer chromatography–bioautography of F31D-CF showed that the compound inhibiting F. oxysporum f. sp. lycopersici growth had an Rf of 0.57. The effective compound in F31D-CF has a molecular weight of > 3000 and is heat stable below 100 °C. Our results suggest that secondary metabolites of isolate F31D have potential for developing a new fungicidal agent against F. oxysporum f. sp. lycopersici.

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Data availability

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank database under Accession No. LC599499.

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Acknowledgements

The authors are thankful to the Faculty of Life and Environmental Science, Shimane University, for financial support to publish this report. This research study was supported and funded by the Project for the Promotion and Enhancement of the Afghan Capacity for Effective Development of the Japan International Cooperation Agency (JICA).

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Authors and Affiliations

  1. Graduate School of Natural Science and Technology, Shimane University, 1060 , Matsue, Shimane, 690-8504, Japan

    Zal Khan Abdullah, Junichi Kihara, Yuri Gondo & Makoto Ueno

  2. Laboratory of Plant Pathology, Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan

    Junichi Kihara, Rattrikorn Ganphung, Yuichiro Yokoyama & Makoto Ueno

  3. Department of Plant Protection, Faculty of Agriculture, Helmand University, Peace Watt, Lashkar Gah, 3901, Helmand, Afghanistan

    Zal Khan Abdullah

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  1. Zal Khan Abdullah
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Correspondence to Makoto Ueno.

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Abdullah, Z.K., Kihara, J., Gondo, Y. et al. Suppressive effect of secondary metabolites from Streptomyces plumbeus isolate F31D against Fusarium oxysporum f. sp. lycopersici, the causal agent of Fusarium wilt of tomato. J Gen Plant Pathol 87, 335–343 (2021). https://doi.org/10.1007/s10327-021-01020-x

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