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Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation


An easy, rapid method has been needed to test the pathogenicity of strains of Ralstonia pseudosolanacearum, the cause of serious bacterial wilt on ginger (Zingiber officinale). When ginger leaves were clip-inoculated with race 4 strains, yellowing and V-shaped lesions developed on leaves, but no lesions formed after inoculation with race 1 strain or on mock-inoculated leaves. Tests of different bacterial concentrations as inoculum showed that the optimal concentration was greater than or equal to 106 cfu/ml. This simple, high-throughput leaf clipping inoculation method provides an efficient, easy method to evaluate pathogenicity of R. pseudosolanacearum race 4 on ginger.

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Part of this research was supported by the grants from the Project of the NARO Bio-Oriented Technology Research Advancement Institution (Research Program on Development of Innovative Technology No. 29014C).

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Correspondence to Kazuhiro Iiyama.

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Iiyama, K., Imamura, M., Inoue, T. et al. Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation. J Gen Plant Pathol 87, 269–272 (2021).

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  • Ralstonia pseudosolanacearum
  • Ginger
  • Virulence
  • Pathogenicity