An easy, rapid method has been needed to test the pathogenicity of strains of Ralstonia pseudosolanacearum, the cause of serious bacterial wilt on ginger (Zingiber officinale). When ginger leaves were clip-inoculated with race 4 strains, yellowing and V-shaped lesions developed on leaves, but no lesions formed after inoculation with race 1 strain or on mock-inoculated leaves. Tests of different bacterial concentrations as inoculum showed that the optimal concentration was greater than or equal to 106 cfu/ml. This simple, high-throughput leaf clipping inoculation method provides an efficient, easy method to evaluate pathogenicity of R. pseudosolanacearum race 4 on ginger.
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Part of this research was supported by the grants from the Project of the NARO Bio-Oriented Technology Research Advancement Institution (Research Program on Development of Innovative Technology No. 29014C).
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Iiyama, K., Imamura, M., Inoue, T. et al. Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation. J Gen Plant Pathol 87, 269–272 (2021). https://doi.org/10.1007/s10327-021-01017-6
- Ralstonia pseudosolanacearum