Abstract
Gray-mold neck rot is an important disease affecting onion (Allium cepa L.) production areas in Japan including Hokkaido, the northernmost region. We examined 10 representative isolates of Botrytis spp. collected from onion bulbs with symptoms of gray-mold neck rot in Hokkaido from 2009 to 2012. We analyzed conidial morphology and the number of nuclei in these isolates. Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis was performed to identify the species. Five of the 10 isolates were identified as B. aclada; the remaining were identified as B. allii. From 2011 to 2017, we collected additional isolates from 50 fields in eight regions of Hokkaido and analyzed them with PCR–RFLP to clarify the distribution of Botrytis spp. Of the total 89 isolates, 63 were identified as B. allii and the remaining as B. aclada. B. allii was isolated from all eight regions of Hokkaido, whereas B. aclada was isolated from six regions, demonstrating the wide distribution of both species in Hokkaido and the tendency for B. allii to dominate. An inoculation test revealed the two species were pathogenic on onion bulbs. This report is the first of B. aclada causing onion gray-mold neck rot in Japan.
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References
Bertolini P, Tian SP (1997) Effect of temperature of production of Botrytis allii conidia on their pathogenicity to harvested white onion bulbs. Plant Pathol 46:432–438
Chilvers MI, du Toit LJ (2006) Detection and identification of Botrytis species associated with neck rot, scape blight, and umbel blight of onion. Plant Health Progress 7:38. https://doi.org/10.1094/PHP-2006-1127-01-DG
Hemmi and Niwa (1939) On Botrytis rots of stored onions. Jpn J Phytopathol 8(4):309–326 ((in Japanese with English summary))
Hwang SK, Lee SY, Back CG, Kang IK, Lee HB, Jung HY, Ohga S (2016) No reported species, Botrytis aclada causing gray mold neck rot disease on onion bulbs in Korea. J Fac Agric Kyushu Univ 61(2):263–266
Iketani SM, Notsu A, Shirai K (2016) Susceptible growth stage of onion to gray-mold neck rot judged by various inoculation periods. Ann Rpt Plant Prot N Jpn 67:108–111 ((In Japanese.))
Matsuo A, Goda K, Furukawa J (1978) Studies on leaf blight of onion by Botrytis spp. 1. On Botrytis spp. isolated from onion leaves. Bull Hyogo Pref Agri Exp Sta 19:85–92 ((in Japanese))
NARO Genebank (2020) Database of plant diseases in Japan. https://www.gene.affrc.go.jp/databases-micro_pl_diseases.php. Accessed 28 May 2020
Nielsen K, Yohalem DS (2001) Origin of a polyploid Botrytis pathogen through interspecific hybridization between Botrytis aclada and B. byssoidea. Mycologia 93:1064–1071
Nielsen K, Justesen AF, Jensen DF, Yohalem DS (2001) Universally primed polymerase chain reaction alleles and internal transcribed spacer restriction fragment length polymorphisms distinguish two subgroups in Botrytis aclada distinct from B. byssoidea. Phytopathology 91:527–533
Nielsen K, Yohalem DS, Jensen DF (2002) PCR detection and RFLP differentiation of Botrytis species associated with neck rot of onion. Plant Dis 86:682–686
Shirane N, Masuko M, Hayashi Y (1989) Light microscopic observation of nuclei and mitotic chromosomes of Botrytis species. Phytopathology 79:728–730
Staats M, van Baarlen P, van Kan JA (2005) Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Mol Biol Evol 22:333–346
Viljanen-Rollinson SLH, Marroni MV, Butler RC (2007) Reduced sensitivity to carbendazim in isolates of Botrytis allii. N Z Plant Protect 60:108–113
Yohalem DS, Nielsen K, Nicolaisen M (2003) Taxonomic and nomenclatural clarification of the onion neck rotting Botrytis species. Mycotaxon 85:175–182
Zhang J, Zhang L, Li GQ, Yang L, Jiang DH, Zhuang WY, Huang HC (2010) Botrytis sinoallii: a new species of the grey mould pathogen on Allium crops in China. Mycoscience 51:421–431
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Notsu, A., Shirai, K., Kodama, F. et al. Distribution of Botrytis isolates associated with onion gray-mold neck rot in Hokkaido, northern Japan. J Gen Plant Pathol 87, 9–15 (2021). https://doi.org/10.1007/s10327-020-00971-x
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DOI: https://doi.org/10.1007/s10327-020-00971-x