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Occurrence of tomato leaf mold caused by novel race 2.4.9 of Cladosporium fulvum in Japan

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Abstract

Leaf mold disease caused by Cladosporium fulvum appeared on tomato cultivars carrying the Cf-9 resistance gene in Nara Prefecture, Japan, in 2018. We inoculated a differential set of tomato cultivar with six isolates of the fungus and identified two isolates as race 2.9 and four as 2.4.9. We also sequenced their Avr and mating-type genes. All Avr alleles have been found previously in Japanese populations of C. fulvum, but the genotypes of both races were new, suggesting that these isolates have newly emerged in Nara Prefecture.

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References

  • den Dunnen JT, Antonarakis SE (2000) Mutation nomenclature extensions and suggestions to describe complex mutations: a discussion. Hum Mutat 15:7–12

    Article  Google Scholar 

  • Dixon MS, Jones DA, Keddie JS, Thomas CM, Harrison K, Jones JDG (1996) The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins. Cell 84:451–459

    Article  CAS  Google Scholar 

  • Dixon MS, Hatzixanthis K, Jones DA, Harrison K, Jones JDG (1998) The tomato Cf-5 disease resistance gene and six homologs show pronounced allelic variation in leucine-rich repeat copy number. Plant Cell 10:1915–1925

    CAS  PubMed  PubMed Central  Google Scholar 

  • Enya J, Ikeda K, Takeuchi T, Horikoshi N, Higashi T, Sakai T, Iida Y, Nishi K, Kubota M (2009) The first occurrence of leaf mold of tomato caused by races 4.9 and 4.9.11 of Passalora fulva (syn. Fulvia fulva) in Japan. J Gen Plant Pathol 75:76–79

    Article  Google Scholar 

  • Iida Y, Iwadate Y, Kubota M, Terami F (2010) Occurrence of a new race 2.9 of leaf mold of tomato in Japan. J Gen Plant Pathol 76:84–86

    Article  Google Scholar 

  • Iida Y, van Hof P, Beenen H, Mesarich C, Kubota M, Stergiopoulos I, Mehrabi R, Notsu A, Fujiwara K, Bahkali A, Abd-Elsalam K, Collemare J, de Wit PJGM (2015) Novel mutations detected in avirulence genes overcoming tomato Cfresistance genes in isolates of a Japanese population of Cladosporium fulvum. PLoS ONE 10:e0123271

    Article  Google Scholar 

  • Jones DA, Thomas CM, Hammond-Kosack KE, Balint-Kurti PJ, Jones JDG (1994) Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science 266:789–793

    Article  CAS  Google Scholar 

  • Kishi K, Abiko K (1976) Studies on the physiological specialization of Cladosporium fulvum Cooke. II. Racial identification of isolates collected from 11 prefectures in Japan from 1971 to 1973 (in Japanese with English abstract). Ann Phytopathol Soc Jpn 42:497–499

    Article  Google Scholar 

  • Kubota M, Iida Y (2019) Tomato fixed lines for identifying races of the leaf mold pathogen (in Japanese). Annu Rep Kansai Plant Prot 61:55–60

    Article  Google Scholar 

  • Kubota M, Morishita M, Iida Y (2015) First occurrence of tomato leaf mold caused by the novel races 2.5.9 and 4.5.9 of Passalora fulva in Japan. J Gen Plant Pathol 81:320–323

    Article  Google Scholar 

  • Li S, Zhao T, Li H, Xu X, Li J (2015) First report of races 2.5 and 2.4.5 of Cladosporium fulvum (syn. Passalora fulva), causal fungus of tomato leaf mold disease in China. J Gen Plant Pathol 81:162–165

    Article  Google Scholar 

  • Stergiopoulos I, de Wit PJGM (2009) Fungal effector proteins. Annu Rev Phytopathol 47:233–263

    Article  CAS  Google Scholar 

  • Stergiopoulos I, de Kock MJD, Lindhout P, de Wit PJGM (2007) Allelic variation in the effector genes of the tomato pathogen Cladosporium fulvum reveals different modes of adaptive evolution. Mol Plant Microbe Interact 20:1271–1283

    Article  CAS  Google Scholar 

  • Takken FLW, Thomas CM, Joosten MHAJ, Golstein C, Westerink N, Hille J, Nijkamp HJJ, de Wit PJGM, Jones JDG (1999) A second gene at the tomato Cf-4 locus confers resistance to Cladosporium fulvum through recognition of a novel avirulence determinant. Plant J 20:279–288

    Article  CAS  Google Scholar 

  • Thomas CM, Jones DA, Parniske M, Harrison K, Balint-Kurti PJ, Hatzixanthis K, Jones JD (1997) Characterization of the tomato Cf-4 gene for resistance to Cladosporium fulvum identifies sequences that determine recognitional specificity in Cf-4 and Cf-9. Plant Cell 9:2209–2224

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We are grateful to Yumiko Higashi and Hisakazu Mogi for their technical assistance. This work was supported by a Grant-in-Aid for Scientific Research from JSPS (17H05022) (Y. Iida).

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Author notes

  1. Kandai Yoshida

    Present address: Nara Prefecture Livestock Division, Noboriohjicho 30, Nara, Japan

  2. Shunsuke Asano

    Present address: Hokubu Agriculture and Forestry Development Center of Nara Prefecture, Manganji 60-1, Yamatokoriyama, Nara, Japan

  3. Hirotoshi Sushida

    Present address: National Agriculture and Food Research Organization, Ikenodai 2, Tsukuba, Ibaraki, Japan

  4. Yuichiro Iida

    Present address: Laboratory of Plant Pathology, Faculty of Agriculture, Setsunan University, Nagaotoge-cho 45-1, Hirakata, Osaka, Japan

Authors and Affiliations

  1. Nara Prefecture Agricultural Research and Development Center, Sakurai, Nara, Japan

    Kandai Yoshida & Shunsuke Asano

  2. National Agriculture and Food Research Organization, Kusawa 360, Tsu, Mie, Japan

    Hirotoshi Sushida & Yuichiro Iida

Authors
  1. Kandai Yoshida
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  2. Shunsuke Asano
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  3. Hirotoshi Sushida
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  4. Yuichiro Iida
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Corresponding author

Correspondence to Yuichiro Iida.

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Yoshida, K., Asano, S., Sushida, H. et al. Occurrence of tomato leaf mold caused by novel race 2.4.9 of Cladosporium fulvum in Japan. J Gen Plant Pathol 87, 35–38 (2021). https://doi.org/10.1007/s10327-020-00963-x

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  • DOI: https://doi.org/10.1007/s10327-020-00963-x

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