Development of a selective medium and antisera for Pseudomonas syringae pv. syringae from seeds of barley and wheat

Abstract

Bacterial black node, a disease of barley and wheat, is caused by Pseudomonas syringae pv. syringae (synonym pv. japonica, hereafter Psj). Here a selective medium, serine-potassium tellurite-based Psj-selective agar (SPTPsjA) was developed to isolate and detect Psj. After 5–7 days on SPTPsjA at 25 °C, characteristic black Psj colonies formed with an efficiency equal to that on potato–peptone–glucose agar. Except for P. viridiflava, P. cichorii, and some strains of P. syringae group bacteria, SPTPsjA either inhibited growth of other phytopathogenic bacteria or enabled their discrimination. To facilitate diagnosis of Psj, we prepared three antisera against three strains; in a slide agglutination test, the antisera reacted to all tested strains of Psj and failed to react to P. viridiflava and P. cichorii although some strains of other P. syringae group bacteria were indistinguishable from Psj with SPTPsjA and antisera. When barley and wheat seeds from fields in which bacterial black node had occurred were placed on SPTPsjA in the dark at 25 °C for 7 days, black colonies grew on the medium around the seeds. Among these strains, only those responsive to the above antisera were pathogenic on barley. These results show that the combination of SPTPsjA and antisera can isolate Psj from seeds of barley and wheat.

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Acknowledgements

We are indebted to Dr. Kenichi Tsuchiya (professor emeritus at Kyushu University) and Dr. Jun-Ichirou Yamaguchi for providing valuable strains, and to Drs. Koji Nishiyama and Takanori Miyoshi for helpful advice. This work was supported by a Grant-in-Aid for Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry from the Ministry of Agriculture, Forestry and Fisheries, Japan (Grant number 25063C).

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Correspondence to Yasuhiro Inoue.

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Kazuhiro Sogou: Retired.

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Mori, M., Sogou, K. & Inoue, Y. Development of a selective medium and antisera for Pseudomonas syringae pv. syringae from seeds of barley and wheat. J Gen Plant Pathol 85, 211–220 (2019). https://doi.org/10.1007/s10327-019-00838-w

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Keywords

  • Bacterial black node
  • Pseudomonas syringae pv. syringae (synonym pv. japonica)
  • Selective medium
  • Antiserum
  • Slide agglutination reaction