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Genomic RNA accumulation of gentian ovary ring-spot virus and raspberry bushy dwarf virus in pollen tubes

  • Viral and Viroid Diseases
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Abstract

The time course of genomic RNA (gRNA) accumulation of gentian ovary ring-spot virus (GORV) and raspberry bushy dwarf virus (RBDV) among total RNAs from germinating pollen grains and elongating pollen grains incubated in germination buffer was assessed by quantitative reverse transcription-polymerase chain reactions. GORV and RBDV gRNAs reached a maximum accumulation among the total RNAs after 3 and 1 h of incubation, respectively. Because the viral gRNAs could have been present in the germination buffer rather than in the pollen grains, GORV and RBDV might replicate and be discharged from the growing pollen tubes.

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Acknowledgements

We thank Prof. K.-T. Sekine, University of the Ryukyus, for providing GORV; Prof. A.O. Jackson, University of California, Berkeley, for his help with improving the manuscript and helpful comments; Prof. M. Watanabe, Tohoku University, for fruitful discussion. This work was supported by JSPS KAKENHI (no. 17K07664).

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Authors and Affiliations

  1. Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka, Iwate, 020-8550, Japan

    Masamichi Isogai, Youhei Suzuki, Takanori Matsudaira & Nobuyuki Yoshikawa

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  1. Masamichi Isogai
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  2. Youhei Suzuki
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  3. Takanori Matsudaira
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  4. Nobuyuki Yoshikawa
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Correspondence to Masamichi Isogai.

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Isogai, M., Suzuki, Y., Matsudaira, T. et al. Genomic RNA accumulation of gentian ovary ring-spot virus and raspberry bushy dwarf virus in pollen tubes. J Gen Plant Pathol 84, 376–380 (2018). https://doi.org/10.1007/s10327-018-0795-2

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  • DOI: https://doi.org/10.1007/s10327-018-0795-2

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