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Pseudomonas syringae pv. alliifistulosi pv. nov., the causal agent of bacterial leaf spot of onions

  • Bacterial and Phytoplasma Diseases
  • Published:
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Abstract

In 1972, bacterial leaf spot of onion (BLSO) was first recorded in Japan by Goto. The pathogen was considered as a pathovar of Pseudomonas syringae specifically causing disease on onion and Welsh onion, but it has not been taxonomically investigated in detail. In 2012 and 2014, a disease suspected as BLSO re-emerged on onion in Shizuoka and Hyogo Prefectures, Japan, respectively. A pathogenic bacterium isolated from the infected onions was thought to be the BLSO agent after preliminary examinations. Strains isolated from BLSO in 1969, 1986, 1987, 2012 and 2014 were characterized and compared with the causal agent of bacterial blight of leek (P. syringae pv. porri), which causes similar symptoms on Allium plants. The result of rep-PCR distinguished the BLSO agent from P. syringae pv. porri. Multilocus sequence analysis on housekeeping genes and hrp genes encoding the type-III secretion system revealed that the strains of the BLSO agent clustered independently of P. syringae pv. porri. The BLSO agent and P. syringae pv. porri also differed in utilization of erythritol, dl-homoserine, glutaric acid and other bacteriological characteristics and caused different reactions on onion, Welsh onions, chives, shallot, rakkyo, leek, garlic and Chinese chive. Thus, the BLSO agent clearly differs from P. syringae pv. porri and is considered to be a new pathovar of P. syringae. The name P. syringae pv. alliifistulosi is proposed with pathotype strain ICMP3414.

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Acknowledgements

We thank Mr. Shinji Nishiguchi (Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, Awaji Agricultural Technology Institute) for kindly supplying samples, Mr. Yoshinobu Ichikawa (Technical Support Division of Faculty of Agriculture, Shizuoka University) for technical assistance in electron microscopy, and Mr. Hajime Haga (Shizuoka Plant Protection Office) for help with collecting samples. We also thank former students of Shizuoka University, Mr. Toyokazu Kubota and Ms. Hanae Miyazawa, for their assistance with many experiments at our laboratory.

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  1. Laboratory of Plant Pathology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Shizuoka, 422-8529, Japan

    Mizue Tsuji & Yuichi Takikawa

  2. National Agriculture and Food Research Organization, Tohoku Agricultural Research Center, 4 Akahira Shimo-kuriyagawa, Morioka, Iwate, 020-0198, Japan

    Mizue Tsuji

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  1. Mizue Tsuji
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Correspondence to Mizue Tsuji.

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Tsuji, M., Takikawa, Y. Pseudomonas syringae pv. alliifistulosi pv. nov., the causal agent of bacterial leaf spot of onions. J Gen Plant Pathol 84, 343–358 (2018). https://doi.org/10.1007/s10327-018-0791-6

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