Simultaneous detection of benzimidazole-resistant strains of Fusarium head blight using the loop-mediated isothermal amplification-fluorescent loop primer method

  • Ryoji Komura
  • Taku Kawakami
  • Kaori Nakajima
  • Hirofumi Suzuki
  • Chiharu Nakashima
Technique
  • 39 Downloads

Abstract

The loop-mediated isothermal amplification (LAMP)-fluorescent loop primer (FLP) method detects genetic polymorphisms by using a LAMP amplicon and measuring the peak temperatures of fluorescence resonance energy transfer between an FLP and a quencher probe, which is specifically hybridized to a sequence including a single nucleotide polymorphism (SNP). In the present study, the LAMP-FLP method was used to detect mutant genotypes F167Y, E198Q, and F200Y in the β2-tubulin gene region of causal pathogens of Fusarium head blight of wheat that result in methyl benzimidazole carbamate (MBC) resistance, proving its usefulness for monitoring strains with SNPs in target regions of MBC resistance.

Keywords

Benzimidazole fungicides Fungicide resistance Fusarium asiaticum Fusarium graminearum Fusarium head blight Loop-mediated isothermal amplification-fluorescent loop primer 

Notes

Acknowledgements

The isolates of Fusarium graminearum and Fusarium asiaticum examined in this study were kindly donated by Dr. H. Suga (Life Science Research Center, Gifu University, Gifu, Japan). This work was partially supported by JSPS KAKENHI Grant Number 17K07837 and Grant-in-Aid by Institute for Fermentation, Osaka.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest. All the experiments undertaken in this study comply with the current laws of Japan.

Ethical standard

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10327_2018_788_MOESM1_ESM.docx (43 kb)
Supplementary material 1 (DOCX 42 KB)

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Graduate School of BioresourcesMie UniversityTsuJapan
  2. 2.Mie Prefecture Agricultural Research InstituteMatsusakaJapan

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