Skip to main content
SpringerLink
Log in

First report of ‘Candidatus Phytoplasma pruni’ infecting cassava in Japan

  • Disease Note
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Phytoplasmas are a threat to cassava production, but have not been found from cassava in Japan. Here, we present the discovery of phytoplasma in asymptomatic plants propagated in Japan. A 16S rRNA gene sequence analysis showed that the phytoplasma was related to ‘Candidatus Phytoplasma pruni’. Analyses based on 16S rRNA, secY, and ribosomal protein gene sequences revealed that the phytoplasma was closely related to phytoplasmas found in cassava in Latin America. Infected plants remained asymptomatic during cultivation for more than 11 months. This is the first molecular evidence of phytoplasma infection in cassava in Japan.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Alvarez E, Pardo JM (2014) First report of hypovirulence between a reovirus and phytoplasma 16SrIII-L associated with frogskin disease of cassava (Manihot esculenta Crantz) (Abstract). Phytopathology 104(Supplement 3):S3.7

    Google Scholar 

  • Alvarez E, Mejía JF, Llano GA, Loke JB (2007) Detection and characterization of a phytoplasma associated with frog skin disease in cassava. Bull Insectol 60:273

    Google Scholar 

  • Alvarez E, Mejía JF, Llano GA, Loke JB, Calari A, Duduk B, Bertaccini A (2009) Characterization of a phytoplasma associated with frogskin disease in cassava. Plant Dis 93:1139–1145

    Article  CAS  PubMed  Google Scholar 

  • Alvarez E, Llano GA, Mejía JF (2012) Cassava diseases in Latin America, Africa and Asia. In: Howeler RH (ed) The cassava handbook: a reference manual based on the Asian regional cassava training course held in Thailand. CIAT, Cali, pp 258–304

    Google Scholar 

  • Alvarez E, Pardo JM, Mejía JF, Bertaccini A, Thanh ND, Hoat TX (2013) Detection and identification of ‘Candidatus Phytoplasma asteris’-related phytoplasmas associated with a witches’ broom disease of cassava in Vietnam. Phytopathogen Mollic 3:77–81

    Article  Google Scholar 

  • Alvarez E, Pardo JM, Truke MJ (2014a) Detection and identification of ‘Candidatus Phytoplasma asteris’-related phytoplasma associated with a witches’ broom disease of cassava in Cambodia (Abstract). Phytopathology 104(Supplement 3):S3.7

    Google Scholar 

  • Alvarez E, Pardo JM, Dufour D, Moreno JL, Alvarez E (2014b) The metabolism of carbohydrates in roots of cassava (Manihot esculenta Crantz) infected with frogskin disease (Abstract). Phytopathology 104(Supplement 3):S3.7

    Google Scholar 

  • Arocha Y, Piñol B, Almeida R, Acosta K, Quiñones M, Zayas T, Varela M, Marrero Y, Boa E, Lucas JA (2009) First report of phytoplasmas affecting organoponic crops in central and eastern Cuba. Plant Pathol 58:793

    Article  Google Scholar 

  • Davis RI, Arocha Y, Jones P, Malau A (2005) First report of the association of phytoplasmas with plant diseases in the territory of Wallis and Futuna. Australas Plant Pathol 34:417–418

    Article  Google Scholar 

  • Davis RE, Zhao Y, Dally EL, Lee I-M, Jomantiene R, Douglas SM (2013) ‘Candidatus Phytoplasma pruni’, a novel taxon associated with X-disease of stone fruits, Prunus spp.: multilocus characterization based on 16S rRNA, secY, and ribosomal protein genes. Int J Syst Evol Microbiol 63:766–776

    Article  PubMed  CAS  Google Scholar 

  • de Souza AN, da Silva FN, Bedendo IP, Carvalho CM (2014) A phytoplasma belonging to a 16SrIII-A subgroup and dsRNA virus associated with cassava frogskin disease in Brazil. Plant Dis 98:771–779

    Article  CAS  PubMed  Google Scholar 

  • Flôres D, Haas IC, Canale MC, Bedendo IP (2013) Molecular identification of a 16SrIII-B phytoplasma associated with cassava witches’ broom disease. Eur J Plant Pathol 137:237–242

    Article  Google Scholar 

  • Goncalves RD, Normanha ES, Boock OJ (eds) (1942) O superbrotamento ou envassouramento da mandioca (in Portuguese). Secretaria de Agricultura, Industria e Comercio, Sao Paulo

    Google Scholar 

  • Jung HY, Sawayanagi T, Kakizawa S, Nishigawa H, Wei W, Oshima K, Miyata S, Ugaki M, Hibi T, Namba S (2003) ‘Candidatus Phytoplasma ziziphi’, a novel phytoplasma taxon associated with jujube witches’-broom disease. Int J Syst Evol Microbiol 53:1037–1041

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Lee I-M, Bottner-Parker KD, Zhao Y, Davis RE, Harrison NA (2010) Phylogenetic analysis and delineation of phytoplasmas based on secY gene sequences. Int J Syst Evol Microbiol 60:2887–2897

    Article  PubMed  Google Scholar 

  • Lozano JC (1992) Overview of integrated control of cassava diseases. Fitopatol Bras 17:18–22

    Google Scholar 

  • Martini M, Lee I-M, Bottner KD, Zhao Y, Botti S, Bertaccini A, Harrison NA, Carraro L, Marcone C, Khan AJ, Osler R (2007) Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. Int J Syst Evol Microbiol 57:2037–2051

    Article  PubMed  CAS  Google Scholar 

  • Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Namba S (1996) Taxonomy of phytoplasmas (in Japanese). Plant Prot 50:152–156

    Google Scholar 

  • Namba S, Oyaizu H, Kato S, Iwanami S, Tsuchizaki T (1993a) Phylogenetic diversity of phytopathogenic mycoplasmalike organisms. Int J Syst Bacteriol 43:461–467

    Article  PubMed  CAS  Google Scholar 

  • Namba S, Kato S, Iwanami S, Oyaizu H, Shiozawa H, Tsuchizaki T (1993b) Detection and differentiation of plant-pathogenic mycoplasmalike organisms using polymerase chain reaction. Phytopathology 83:786–791

    Article  CAS  Google Scholar 

  • Oliveira SAS, Abreu EFM, Araújo TS, Oliveira EJ, Andrade EC, Garcia JMP, Álvarez E (2014) First report of a 16SrIII-L phytoplasma associated with frogskin disease in cassava (Manihot esculenta Crantz) in Brazil. Plant Dis 98:153

    Article  PubMed  Google Scholar 

  • Pineda B, Jayasinghe U, Lozano JC (1983) La enfermedad “cuero de sapo” en yuca (Manihot esculenta Crantz). ASIAVA 4:10–12

    Google Scholar 

  • Takinami Y, Maejima K, Takahashi A, Keima T, Shiraishi T, Okano Y, Komatsu K, Oshima K, Namba S (2013) First report of ‘Candidatus Phytoplasma asteris’ infecting hydrangea showing phyllody in Japan. J Gen Plant Pathol 79:209–213

    Article  Google Scholar 

  • Téllez LC, Pardo JM, Zacher M, Torres A, Alvarez E (2016) First report of a 16SrIII phytoplasma associated with frogskin disease in cassava (Manihot esculenta) in Paraguay. Plant Dis 100:1492

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Hiroaki Koinuma, Akio Miyazaki, Renya Wakaki, Yuji Fujimoto, Nozomu Iwabuchi, Takamichi Nijo, Yugo Kitazawa, Toshiro Shigaki, Kensaku Maejima, Yasuyuki Yamaji & Shigetou Namba

Authors
  1. Hiroaki Koinuma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akio Miyazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Renya Wakaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuji Fujimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nozomu Iwabuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Takamichi Nijo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yugo Kitazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Toshiro Shigaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Kensaku Maejima
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Yasuyuki Yamaji
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Shigetou Namba
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shigetou Namba.

Ethics declarations

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflicts of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Koinuma, H., Miyazaki, A., Wakaki, R. et al. First report of ‘Candidatus Phytoplasma pruni’ infecting cassava in Japan. J Gen Plant Pathol 84, 300–304 (2018). https://doi.org/10.1007/s10327-018-0787-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-018-0787-2

Keywords

Search

Navigation