Journal of General Plant Pathology

, Volume 84, Issue 3, pp 202–207 | Cite as

Ampeloviruses associated with incomplete flower syndrome and leaf-edge necrosis in Japanese apricot

  • Ryoji Nakaune
  • Tomoaki Takeda
  • Koji Numaguchi
  • Eiko Nakazono-Nagaoka
  • Takashi Fujikawa
Viral and Viroid Diseases


Japanese apricot (Prunus mume Sieb. et Zucc.) trees produce popular fruits and are also used in Japan as ornamental flowering trees. Since the 1980s, graft-transmissible symptoms (e.g., incomplete flowers and leaf-edge necrosis) have been observed on ‘Nanko’ trees in Wakayama Prefecture. We here describe the detection of viruses associated with these symptoms in Japanese apricot trees using DECS analysis (i.e., double-stranded RNA [dsRNA] isolation, exhaustive amplification, cloning, and sequencing). We isolated dsRNA from symptomatic flowers, including dsRNA of plum bark necrosis stem pitting-associated virus (PBNSPaV), little cherry virus 2 (LChV-2), and a Luteovirus-like sequence. The reverse transcription polymerase chain reaction results suggested that PBNSPaV and LChV-2 may induce the development of incomplete flowers and leaf-edge necrosis. In contrast, the Luteovirus-like sequence is apparently unrelated to these symptoms. Additional investigations are necessary to clarify the relationship between the symptoms and viral infections.


Japanese apricot Incomplete flower Leaf-edge necrosis Double-stranded RNA Ampelovirus 



The authors thank the growers for their cooperation and providing access to trees used in this study. They also thank Mr. Y. Tanimoto, Mr. Y. Akagi and Dr. Y. Tsuchida (Wakayama Fruit Tree Experiment Station) for helpful comments and suggestions on the study, and Dr. Y. Tomitaka (Central Region Agricultural Research Center, NARO) for providing the CMV sample. This study was funded by the NARO.


  1. Abou Ghanem-Sabanadzovic N, Mahboubi M, Di Terlizzi B, Sabanadzovic S, Savino V, Uyemoto JK, Martelli GP (2001) Molecular detection of a closterovirus associated with apricot stem pitting in southern Italy. J Plant Pathol 83:125–132Google Scholar
  2. Al Rwahnih M, Uyemoto JK, Falk BW, Rowhani A (2007) Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Arch Virol 152:2197–2206CrossRefPubMedGoogle Scholar
  3. Boscia D, Myrta A, Uyemoto JK (2011) Plum bark necrosis stem pitting-associated virus. In: Hadidi A, Barba M, Candresse T, Jelkmann W (eds) Virus and virus-like diseases of pome and stone fruits. APS Press, St. Paul, pp 177–179Google Scholar
  4. Bouani A, Minafra A, Alami I, Zemzami M, Myrta A (2004) First report of plum bark necrosis stem pitting-associated virus in Morocco. J Plant Pathol 86:91Google Scholar
  5. Eastwell KC, Bernardy MG (2001) Partial characterization of a closterovirus associated with apple mealybug-transmitted little cherry disease in North America. Phytopathology 91:268–273CrossRefPubMedGoogle Scholar
  6. Iemura H, Shimazu K, Natsumi K (1995) Occurrence of a graft transmissible disease of mume (abstract in Japanese). Ann Phytopathol Soc Jpn 61:631Google Scholar
  7. Jelkmann W, Eastwell KC (2011) Little cherry virus-1 and 2. In: Hadidi A, Barba M, Candresse T, Jelkmann W (eds) Virus and virus-like diseases of pome and stone fruits. APS Press, St. Paul, pp 153–159Google Scholar
  8. Kansako M, Iemura H, Natsumi K, Shimazu K (2000) Studies on Japanese apricot leaf-edge necrosis disease (in Japanese with English summary). Ann Rep Wakayama Res Center of Agriculture. Forestry Fish 1:67–78Google Scholar
  9. Kobayashi K, Tomita R, Sakamoto M (2009) Recombinant plant dsRNA-binding protein as an effective tool for the isolation of viral replicative form dsRNA and universal detection of RNA viruses. J Gen Plant Pathol 75:87–91CrossRefGoogle Scholar
  10. Kurihara J, Tsuda S, Kirita M, Natsuaki KT, Tomaru K (2005) Cucumber mosaic virus isolated from Prunus mume (Japanese apricot) belongs to subgroup I but is distinct in serology. J Agric Sci Tokyo Univ Agric 50:64–70Google Scholar
  11. Kurihara J, Tomaru K, Otsubo T, Natsuaki KT, Arimoto Y (2006) Mume leaf margin necrosis, a new disease of Japanese apricot caused by multiple infection with Cucumber mosaic virus and Prunus necrotic ringspot related Ilarvirus. J Agric Sci Tokyo Univ Agric 50:95–105Google Scholar
  12. Lenz O, Přibylová J, Fránová J, Koloniuk I, Špak J (2017) Identification and characterization of a new member of the genus Luteovirus from cherry. Arch Virol 162:587–590CrossRefPubMedGoogle Scholar
  13. MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226CrossRefGoogle Scholar
  14. MAFF (2016) Statistical tables, VII Crops: part 6, Fruits and nuts. The 90th statistical yearbook of Ministry of Agriculture, Forestry and Fisheries. (accessed 15 June 2017)
  15. Marais A, Faure C, Couture C, Bergey B, Gentit P, Candresse T (2014) Characterization by deep sequencing of divergent Plum bark necrosis stem pitting-associated virus (PBNSPaV) isolates and development of a broad-spectrum PBNSPaV detection assay. Phytopathology 104:660–666CrossRefPubMedGoogle Scholar
  16. Marini DB, Zhang YP, Rowhani A, Uyemoto JK (2002) Etiology and host range of a Closterovirus associated with plum bark necrosis-stem pitting disease. Plant Dis 86:415–417CrossRefGoogle Scholar
  17. Mekuria TA, Smith TJ, Beers E, Watson GW, Eastwell KC (2013) First report of transmission of Little cherry virus 2 to sweet cherry by Pseudococcus maritimus (Ehrhorn) (Hemiptera: Pseudococcidae). Plant Dis 97:851CrossRefGoogle Scholar
  18. Nakaune R, Nakano M (2006) Efficient methods for sample processing and cDNA synthesis by RT-PCR for the detection of grapevine viruses and viroids. J Virol Method 134:244–249CrossRefGoogle Scholar
  19. Ohtubo T, Kurihara J, Sakakibara M, Tomaru K (2002) Confirmation of symptom reproduction in mume leaf margin necrosis disease on Prunus mume (cv. Nanko) by sap inoculation with causal two viruses, Cucumber mosaic virus and Prunus necrotic ringspot-like viruses (in Japanese with English summary). J Agri Sci Tokyo Univ Agric 47:11–16Google Scholar
  20. Okuda M, Kubota K, Onuki M (2007) Development of RT-PCR assay using a primer cocktail for eight virus species infecting melon and cucumber (in Japanese with English summary). Kyushu Pl Prot Res 53:9–13CrossRefGoogle Scholar
  21. Otsubo T, Harano H, Natsuaki K, Tomaru K (1991) Chakasu disorder of Japanese apricot (in Japanese). J Japan Soc Hort Sci 60(Suppl 1):164–165Google Scholar
  22. Raine J, McMullen RD, Forbes AR (1986) Transmission of the agent causing little cherry disease by the apple mealybug Phenacoccus aceris and the dodder Cuscuta lupuliformis. Can J Plant Pathol 8:6–11CrossRefGoogle Scholar
  23. Rott ME, Jelkmann W (2001) Detection and partial characterization of a second closterovirus associated with little cherry disease, Little cherry virus-2. Phytopathology 91:261–267CrossRefPubMedGoogle Scholar
  24. Shimazu K, Natsumi K (1989) Tests of causal factors of ‘mume chagasu’ syndrome (in Japanese). Ann Rep Wakayama Fruit Tree Exp Sta 114–115Google Scholar
  25. Tzanetakis IE, Martin RR (2008) A new method for extraction of double-stranded RNA from plants. J Virol Method 149:167–170CrossRefGoogle Scholar
  26. Untiveros M, Perez-Egusquiza Z, Clover G (2010) PCR assays for the detection of members of the genus Ilarvirus and family Bromoviridae. J Virol Method 165:97–104CrossRefGoogle Scholar
  27. Wetzel T, Candresse T, Ravelonandro M, Dunez J (1991) A polymerase chain reaction assay adapted to plum pox potyvirus detection. J Virol Method 33:355–365CrossRefGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Ryoji Nakaune
    • 1
  • Tomoaki Takeda
    • 2
  • Koji Numaguchi
    • 2
  • Eiko Nakazono-Nagaoka
    • 1
  • Takashi Fujikawa
    • 1
  1. 1.Institute of Fruit Tree and Tea ScienceNational Agriculture and Food Research Organization (NARO)TsukubaJapan
  2. 2.Japanese Apricot LaboratoryWakayama Fruit Tree Experiment StationMinabeJapan

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