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Journal of General Plant Pathology

, Volume 84, Issue 3, pp 202–207 | Cite as

Ampeloviruses associated with incomplete flower syndrome and leaf-edge necrosis in Japanese apricot

  • Ryoji Nakaune
  • Tomoaki Takeda
  • Koji Numaguchi
  • Eiko Nakazono-Nagaoka
  • Takashi Fujikawa
Viral and Viroid Diseases
  • 106 Downloads

Abstract

Japanese apricot (Prunus mume Sieb. et Zucc.) trees produce popular fruits and are also used in Japan as ornamental flowering trees. Since the 1980s, graft-transmissible symptoms (e.g., incomplete flowers and leaf-edge necrosis) have been observed on ‘Nanko’ trees in Wakayama Prefecture. We here describe the detection of viruses associated with these symptoms in Japanese apricot trees using DECS analysis (i.e., double-stranded RNA [dsRNA] isolation, exhaustive amplification, cloning, and sequencing). We isolated dsRNA from symptomatic flowers, including dsRNA of plum bark necrosis stem pitting-associated virus (PBNSPaV), little cherry virus 2 (LChV-2), and a Luteovirus-like sequence. The reverse transcription polymerase chain reaction results suggested that PBNSPaV and LChV-2 may induce the development of incomplete flowers and leaf-edge necrosis. In contrast, the Luteovirus-like sequence is apparently unrelated to these symptoms. Additional investigations are necessary to clarify the relationship between the symptoms and viral infections.

Keywords

Japanese apricot Incomplete flower Leaf-edge necrosis Double-stranded RNA Ampelovirus 

Notes

Acknowledgements

The authors thank the growers for their cooperation and providing access to trees used in this study. They also thank Mr. Y. Tanimoto, Mr. Y. Akagi and Dr. Y. Tsuchida (Wakayama Fruit Tree Experiment Station) for helpful comments and suggestions on the study, and Dr. Y. Tomitaka (Central Region Agricultural Research Center, NARO) for providing the CMV sample. This study was funded by the NARO.

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Ryoji Nakaune
    • 1
  • Tomoaki Takeda
    • 2
  • Koji Numaguchi
    • 2
  • Eiko Nakazono-Nagaoka
    • 1
  • Takashi Fujikawa
    • 1
  1. 1.Institute of Fruit Tree and Tea ScienceNational Agriculture and Food Research Organization (NARO)TsukubaJapan
  2. 2.Japanese Apricot LaboratoryWakayama Fruit Tree Experiment StationMinabeJapan

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