Journal of General Plant Pathology

, Volume 84, Issue 1, pp 58–64 | Cite as

Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

  • Kirara Saito
  • Megumi Watanabe
  • Hidenori Matsui
  • Mikihiro Yamamoto
  • Yuki Ichinose
  • Kazuhiro Toyoda
  • Akira Kawaguchi
  • Yoshiteru Noutoshi
Disease Control


Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.


Rhizobium vitis Crown gall disease Biological control agent Antitumorigenic bacterial strain 



The bacterial strains were kindly provided by Okayama Prefecture in Japan via the dedicated arrangement of a material transfer agreement by Mr. Koji Tanina. This research was supported by KAKENHI Grant 17H03778 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A.K. and Y.N., and basic funds from Okayama University to Y.N. We also thank Honokai for providing a scholarship to K.S.

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interests.


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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Graduate School of Environmental and Life ScienceOkayama UniversityOkayamaJapan
  2. 2.Western Region Agricultural Research CenterNational Agricultural and Food Research Organization (NARO)FukuyamaJapan

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