Journal of General Plant Pathology

, Volume 84, Issue 1, pp 65–69 | Cite as

First report of pileus rot disease on cultivated Morchella importuna caused by Diploöspora longispora in China

  • Peixin He
  • Congcong Li
  • Yingli Cai
  • Ya Zhang
  • Yinbing Bian
  • Wei LiuEmail author
Disease Note


In March 2013, pileus rot disease was first observed on cultivated Morchella importuna. Infected ascomata were covered by white, velvety mycelia mainly on the pileus, and the infection resulted in malformed fruiting bodies. The causal pathogen was identified as Diploöspora longispora based on its morphology and the internal transcribed spacer of its ribosomal DNA sequences. After inoculation of young ascomata with the isolates, the original symptoms were reproduced, and the same pathogen was reisolated from diseased ascomata. This is the first report of pileus rot disease on morels caused by D. longisporain China.


Diploöspora longispora Morchella importuna Morel Pileus rot disease 


  1. Castañeda R (1987) Fungi cubenses II. Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro Humboldt”. Academia de Ciencas de Cuba, la Havana, CubaGoogle Scholar
  2. Delaye L, García-Guzmán G, Heil M (2013) Endophytes versus biotrophic and necrotrophic pathogens—are fungal lifestyles evolutionarily stable traits? Fungal Divers 60:125–135CrossRefGoogle Scholar
  3. Du X, Zhao Q, Yang Z (2014) Diversity, evolutionary history and cultivation of morels: a review (in Chinese with English abstract). Mycosystema 33:183–197Google Scholar
  4. Gryzenhout M, Jami F, Slippers B, Wingfield BD, Wingfield MJ (2009) Botryosphaeriaceae as endophytes and pathogens of trees in South Africa. S Afr J Bot 75:403CrossRefGoogle Scholar
  5. Guo MP, Chen K, Wang GZ, Bian YB (2016) First report of stipe rot disease on Morchella importuna caused by Fusarium incarnatumF. equiseti species complex in China. Plant Dis 100:2530CrossRefGoogle Scholar
  6. He P, Liu W, Cai Y, He X (2015) Strain identification and phylogenetic analysis of cultivated and wild strains of Morchella belonging to Elata Clade in China (in Chinese with English abstract). J Light Ind 30:26–29Google Scholar
  7. He X-L, Peng W-H, Miao R-Y, Tang J, Chen Y, Liu L-X, Wang D, Gan B-C (2017) White mold on cultivated morels caused by Paecilomyces penicillatus. FEMS Microbiol Lett 364:fnx037CrossRefGoogle Scholar
  8. Hibbett DS, Ohman A, Glotzer D, Nuhn M, Kirk P, Nilsson RH (2011) Progress in molecular and morphological taxon discovery in Fungi and options for formal classification of environmental sequences. Fungal Biol Rev 25:38–47CrossRefGoogle Scholar
  9. Inglis PW, Tigano MS (2006) Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS sequences. Genet Mol Biol 29:132–136CrossRefGoogle Scholar
  10. Junker C, Draeger S, Schulz B (2012) A fine line—endophytes or pathogens in Arabidopsis thaliana. Fungal Ecol 5:657–662CrossRefGoogle Scholar
  11. Kirk PM, Cannon PF, Minter DW, Stalpers JA (2008) Ainsworth & Bisby’s dictionary of the fungi, 10th edn. CABI Europe, WallingfordCrossRefGoogle Scholar
  12. Linder DL, Banik MT (2011) Intragenomic variation in the ITS rDNA region obscures phylogenetic relationships and inflates estimates of operational taxonomic units in genus Laetiporus. Mycologia 103:731–740CrossRefGoogle Scholar
  13. Luangsa-ard JJ, Hywel-Jones NL, Samson RA (2004) The polyphyletic nature of Paecilomyces sensu lato based on 18S-generated rDNA phylogeny. Mycologia 96:773–780CrossRefPubMedGoogle Scholar
  14. Matsushima T (1975) Icones microfungorum a matsushima lectorum. Nippon Printing & Publishing Co. Ltd., KobeGoogle Scholar
  15. Moncalvo JM, Nilsson RH, Koster B, Dunham SM, Bernauer T, Matheny PB, Porter TM, Margaritescu S, Weiß M, Garnica S, Danell E, Langer G, Langer E, Larsson E, Larsson KH, Vilgalys R (2006) The cantharelloid clade: dealing with incongruent gene trees and phylogenetic reconstruction methods. Mycologia 98:937–948CrossRefPubMedGoogle Scholar
  16. O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116CrossRefPubMedGoogle Scholar
  17. Padhi S, Panda MK, Das D, Tayung K (2016) ITS2 RNA secondary structure analysis reveals close affinity between endophytic and pathogenic fungi: A case study in Fusarium species. Ann Microbiol 66:625–633CrossRefGoogle Scholar
  18. Sakalidis ML, Hardy GESJ., Burgess TI (2011) Endophytes as potential pathogens of the baobab species Adansonia gregorii: a focus on the Botryosphaeriaceae. Fungal Ecol 4:1–14CrossRefGoogle Scholar
  19. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA109:6241–6246CrossRefGoogle Scholar
  20. Slippers B, Wingfield MJ (2007) Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biol Rev 21:90–106CrossRefGoogle Scholar
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  22. Tanney JB, Nguyen HDT, Pinzari F, Seifert KA (2015) A century later: rediscovery, culturing and phylogenetic analysis of Diploöspora rosea, a rare onygenalean hyphomycete. Antonie Leeuwenhoek 108:1023–1035CrossRefPubMedGoogle Scholar
  23. Watson P (1955) Calacarisporium arbuscula living as an endophyte in apparently healthy sporophores of Russula and Lactarius. Trans Br Mycol Soc 38:409–414CrossRefGoogle Scholar
  24. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA et al (eds) PCR protocols: a guide to methods and applications. Academic, San Diego, pp 315–322Google Scholar
  25. Xie J, Fu Y, Jiang D, Li G, Huang J, Li B, Hsiang T, Peng Y (2008) Intergeneric transfer of ribosomal genes between two fungi. BMC Evol Biol 8:87CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Peixin He
    • 1
    • 2
  • Congcong Li
    • 1
  • Yingli Cai
    • 3
  • Ya Zhang
    • 4
  • Yinbing Bian
    • 3
  • Wei Liu
    • 1
    • 3
    Email author
  1. 1.School of Food and Biological EngineeringZhengzhou University of Light IndustryZhengzhouChina
  2. 2.Collaborative Innovation Center of Food Production and Safety, Henan ProvinceZhengzhouChina
  3. 3.Institute of Applied MycologyHuazhong Agricultural UniversityWuhanChina
  4. 4.SichuanDelilongAgricultural Science and Technology Co., Ltd.ChengduChina

Personalised recommendations