Skip to main content
SpringerLink
Log in

Suppression of bacterial wilt of tomato by soil amendment with mushroom compost containing Bacillus amyloliquefaciens IUMC7

  • Disease Control
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Bacillus amyloliquefaciens IUMC7 and its culture supernatant, which we previously found to suppress Fusarium wilt, were found here to have antimicrobial activity against Ralstonia solanacearum. In in vivo experiments, mushroom compost inoculated with IUMC7 significantly reduced disease severity in tomato plants over that in control soils. The R. solanacearum population decreased in soil inoculated with IUMC7. A TLC–bioautography assay showed that one of the antimicrobial substances produced by IUMC7 is likely an iturin-like lipopeptide. These results suggest that these antimicrobial compounds are responsible for disease suppression and that mushroom compost containing IUMC7 has potential as a biocontrol product.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Arguelles-Arias A, Ongena M, Halimi B, Lara Y, Brans A, Joris B, Fickers P (2009) Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens. Microb Cell Fact 8:63

    Article  PubMed  PubMed Central  Google Scholar 

  • Arrebola E, Sivakumar D, Bacigalupo R, Korsten L (2010) Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases. Crop Prot 29:369–377

    Article  CAS  Google Scholar 

  • Bailey KL, Lazarovits G (2003) Suppressing soil-borne diseases with residue management and organic amendments. Soil Till Res 72:169–180

    Article  Google Scholar 

  • Bonanomi G, Antignani V, Capodilupo M, Scala F (2010) Identifying the characteristics of organic soil amendments that suppress soilborne plant diseases. Soil Biol Biochem 42:136–144

    Article  CAS  Google Scholar 

  • Choudhary DK, Johri BN (2009) Interactions of Bacillus spp. and plants—with special reference to induced systemic resistance (ISR). Microbiol Res 164:493–513

    Article  CAS  PubMed  Google Scholar 

  • Gamliel A, Austerweil M, Kritzman G (2000) Non-chemical approach to soilborne pest management—organic amendments. Crop Prot 19:847–853

    Article  Google Scholar 

  • Gong M, Wang JD, Zhang J, Yang H, Lu XF, Pei Y, Cheng JQ (2006) Study of the antifungal ability of Bacillus subtilis strain PY-1 in vitro and identification of its antifungal substance (iturin A). Acta Biochim Biophys Sin 38:233–240

    Article  CAS  PubMed  Google Scholar 

  • Handelsman J, Stabb EV (1996) Biocontrol of soilborne plant pathogens. Plant Cell 8:1855–1869

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hara H, Ono K (1983) Ecological studies on the bacterial wilt of tobacco, caused by Pseudomonas solanacearum. In: Smith EF (ed.) A selective medium for isolation and detection of P. solanacearum (in Japanese). Bull Okayama Tob Exp Stn, vol 42, pp 127–138

  • Hayward AC (1991) Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol 29:65–87

    Article  CAS  PubMed  Google Scholar 

  • Ji X, Lu G, Gai Y, Zheng C, Mu Z (2008) Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain. FEMS Microbiol Ecol 65:565–573

    Article  CAS  PubMed  Google Scholar 

  • Lemessa F, Zeller W (2007) Screening rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia. Biol Control 42:336–344

    Article  Google Scholar 

  • Li SB, Fang M, Zhou RC, Huang J (2012) Characterization and evaluation of the endophyte Bacillus B014 as a potential biocontrol agent for the control of Xanthomonas axonopodis pv. dieffenbachiae—induced blight of Anthurium. Biol Control 63:9–16

    Article  CAS  Google Scholar 

  • Mari M, Guizzardi M, Brunelli M, Folchi A (1996) Postharvest biological control of grey mould (Botrytis cinerea Pers.:Fr.) on fresh-market tomatoes with Bacillus amyloliquefaciens. Crop Prot 15:699–705

    Article  Google Scholar 

  • Romero D, de Vincente A, Rakotoaly RH, Dufour SE, Veening J-W, Arrebola E, Cazorla FM, Kuipers OP, Paquot M, Pérez-García A (2007) The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Mol Plant Microbe Interact 20:430–440

    Article  CAS  PubMed  Google Scholar 

  • Sotoyama K, Akutsu K, Nakajima M (2016) Biological control of Fusarium wilt by Bacillus amyloliquefaciens IUMC7 isolated from mushroom compost. J Gen Plant Pathol 82:105–109

    Article  CAS  Google Scholar 

  • Stein T (2005) Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol 56:845–857

    Article  CAS  PubMed  Google Scholar 

  • Tan S, Dong Y, Liao H, Huang J, Song S, Xu Y, Shen Q (2013) Antagonistic bacterium Bacillus amyloliquefaciens induces resistance and controls the bacterial wilt of tomato. Pest Manag Sci 69:1245–1252

    CAS  PubMed  Google Scholar 

  • Thasana N, Prapagdee B, Rangkadilok N, Sallabhan R, Aye SL, Ruchirawat S, Loprasert S (2010) Bacillus subtilis SSE4 produces subtulene A, a new lipopeptide antibiotic possessing an unusual C15 unsaturated β-amino acid. FEBS Lett 584:3209–3214

    Article  CAS  PubMed  Google Scholar 

  • Wang X, Liang G (2014) Control efficacy of an endophytic Bacillus amyloliquefaciens strain BZ6-1 against peanut bacterial wilt, Ralstonia solanacearum. BioMed Res Int 2014:465435

    PubMed  PubMed Central  Google Scholar 

  • Yu GY, Sinclair JB, Hartman GL, Bertagnolli BL (2002) Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biol Biochem 34:955–963

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank the College of Agriculture at Ibaraki University for funding this research.

Author information

Authors and Affiliations

  1. Laboratory of Plant Pathology, College of Agriculture, Ibaraki University, Ami-machi, Ibaraki, 300-0393, Japan

    Kou Sotoyama, Katsumi Akutsu & Masami Nakajima

  2. United Graduate School of Agricultural Science Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Kou Sotoyama

Authors
  1. Kou Sotoyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katsumi Akutsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masami Nakajima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masami Nakajima.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sotoyama, K., Akutsu, K. & Nakajima, M. Suppression of bacterial wilt of tomato by soil amendment with mushroom compost containing Bacillus amyloliquefaciens IUMC7. J Gen Plant Pathol 83, 51–55 (2017). https://doi.org/10.1007/s10327-016-0690-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-016-0690-7

Keywords

Search

Navigation