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Multiplex polymerase chain reaction discriminates which eggplant isolates of Corynespora cassiicola are virulent to sweet pepper

  • Fungal Diseases
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Abstract

Some isolates of Corynespora cassiicola from eggplant leaves with black blight can also cause Corynespora blight on sweet pepper. To differentiate eggplant isolates differing in virulence to sweet pepper, we first created two sets of primers based on nucleotide sequences of DNA fragments specific to virulent and to avirulent isolates, respectively, in a random amplified polymorphic DNA analysis using two random primers. A multiplex polymerase chain reaction using the two primer sets allowed us to discriminate isolates virulent to sweet pepper from among eggplant isolates.

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References

  • Conner KN, Hagan AK, Zhang L (2013) First report of Corynespora cassiicola-incited target spot on cotton in Alabama. Plant Dis 97:1379

    Article  Google Scholar 

  • Dixon LJ, Schlub RL, Pernezny K, Datnoff LE (2009) Host specialization and phylogenetic diversity of Corynespora cassiicola. Phytopathology 99:1015–1027

    Article  CAS  PubMed  Google Scholar 

  • Jiménez-Gasco MM, Milgroom MG, Jiménez-Diaz RM (2002) Gene genealogies support Fusarium oxysporum f. sp. ciceris as a monophyletic group. Plant Pathol 51:72–77

    Article  Google Scholar 

  • Luo CX, Yin LF, Koyanagi S, Farman ML, Kusaba M, Yaegashi H (2005) Genetic mapping and chromosomal assignment of Magnaporthe oryzae avirulence genes AvrPik, AvrPiz, and AvrPiz-t controlling cultivar specificity on rice. Phytopathology 95:640–647

    Article  CAS  PubMed  Google Scholar 

  • Park JH, Park MJ, Lee SH, Lee CK, Shin HD (2013) First report of Corynespora leaf spot on beach vitex caused by Corynespora cassiicola in Korea. Plant Dis 97:1512

    Article  Google Scholar 

  • Penner GA, Bush A, Wise R, Kim W, Domier L, Kasha K, Laroche A, Scoles G, Molnar SJ, Fedak G (1993) Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. Genome Res 2:341–345

    Article  CAS  Google Scholar 

  • Qi YX, Zhang X, Pu JJ, Liu XM, Lu Y, Zhang H, Zhang HQ, Lv YC, Xie YX (2011) Morphological and molecular analysis of genetic variability within isolates of Corynespora cassiicola from different hosts. Eur J Plant Pathol 130:83–95

    Article  Google Scholar 

  • Shimomoto Y, Adachi R, Morita Y, Yano K, Kiba A, Hikichi Y, Takeuchi S (2008) Corynespora blight of sweet pepper (Capsicum annuum) caused by Corynespora cassiicola (Berk. and Curt.) Wei. J Gen Plant Pathol 74:335–337

    Article  Google Scholar 

  • Shimomoto Y, Morita Y, Takeuchi S, Hikichi Y, Kiba A, Sato T (2011a) Reidentification of the causal fungus of black blight of eggplant (in Japanese with English summary). Jpn J Phytopathol 77:105–108

    Article  Google Scholar 

  • Shimomoto Y, Sato T, Hojo H, Morita Y, Takeuchi S, Mizumoto H, Kiba A, Hikichi Y (2011b) Pathogenic and genetic variation among isolates of Corynespora cassiicola in Japan. Plant Pathol 60:253–260

    Article  CAS  Google Scholar 

  • Silva WPK, Multani DS, Deverall BJ, Lyon BR (1995) RFLP and RAPD analyses in the identification and differentiation of isolates of the leaf spot fungus Corynespora cassiicola. Aust J Bot 43:609–618

    Article  CAS  Google Scholar 

  • Silva WPK, Deverall BJ, Lyon BR (1998) Molecular, physiological and pathological characterization of Corynespora leaf spot fungi from rubber plantations in Sri Lanka. Plant Pathol 47:267–277

    Article  CAS  Google Scholar 

  • Silva WPK, Karunanayake EH, Wijesundera RLC, Priyanka UMS (2003) Genetic variation in Corynespora cassiicola: a possible relationship between host origin and virulence. Mycol Res 107:567–571

    Article  CAS  PubMed  Google Scholar 

  • Taylor JW, Jacobson DJ, Fisher MC (1999) The evolution of asexual fungi: reproduction, speciation and classification. Annu Rev Phytopathol 37:197–246

    Article  CAS  PubMed  Google Scholar 

  • Ye YF, Jiang N, Fu G, Liu W, Hu FY, Liu LH, Miao JH (2013) First report of Corynespora cassiicola causing leaf spot on Akebia trifoliata. Plant Dis 97:1659

    Article  Google Scholar 

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Acknowledgments

We thank Dr. Makoto Ueno, Faculty of Life and Environmental Sciences, Shimane Univercity, and Koji Tanina, Research Institute for Agriculture, Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries, for their gift of C. cassiicola isolates.

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Authors and Affiliations

  1. Kochi Agricultural Research Center, 1100 Hataeda, Nankoku, Kochi, 783-0023, Japan

    Yoshifumi Shimomoto

  2. Laboratory of Plant Pathology and Biotechnology, Kochi University, 200 Monobe, Nankoku, Kochi, 783-8502, Japan

    Akinori Kiba & Yasufumi Hikichi

Authors
  1. Yoshifumi Shimomoto
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  2. Akinori Kiba
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  3. Yasufumi Hikichi
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Corresponding author

Correspondence to Yoshifumi Shimomoto.

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Shimomoto, Y., Kiba, A. & Hikichi, Y. Multiplex polymerase chain reaction discriminates which eggplant isolates of Corynespora cassiicola are virulent to sweet pepper. J Gen Plant Pathol 81, 226–231 (2015). https://doi.org/10.1007/s10327-015-0584-0

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  • DOI: https://doi.org/10.1007/s10327-015-0584-0

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