Abstract
Here we addressed the involvement of histone methylation at H3 lysine 4 (H3K4me) in substrate-dependent activation of cell-wall-degrading enzymes (CWDEs) in Magnaporthe oryzae. Transcriptional activation of 36 putative CWDEs including cellobiohydrolases, endoglucanases, β-glucosidases, and endoxylanases was assessed by qRT-PCR. Among the CWDE genes, 16 were upregulated during infection and also in media containing their respective substrates with an exception. Interestingly, the substrate-dependent activation of most CWDE genes was severely impaired in a deletion mutant of MoSET1, histone H3K4 methyltransferase in M. oryzae, suggesting that MoSET1 directly or indirectly regulates a wide range of CWDE genes.
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Acknowledgments
This work was supported in part by a Grant-in-Aid for Scientific Research (B) (22380031 and 25292028) from the Japan Society for the Promotion of Science and by a Grant-in-Aid from the Hyogo Science and Technology Association.
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K. T. M. Pham and H. H. Nguyen are the two authors contributed equally to this work.
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Pham, K.T.M., Nguyen, H.H., Murai, T. et al. Histone H3K4 methyltransferase globally regulates substrate-dependent activation of cell-wall-degrading enzymes in Magnaporthe oryzae . J Gen Plant Pathol 81, 127–130 (2015). https://doi.org/10.1007/s10327-014-0570-y
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DOI: https://doi.org/10.1007/s10327-014-0570-y