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Overexpression of OsHAP2E for a CCAAT-binding factor confers resistance to Cucumber mosaic virus and Rice necrosis mosaic virus

  • Viral and Viroid Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

HAP (CBF/NF-Y) transcription factors have important functions in regulating plant growth, development, and stress responses. In this study, we examined whether the endogenous gene OsHAP2E and the GUS transgene driven by the promoter of OsHAP2E respond to virus infection. RT-PCR analyses showed OsHAP2E expression was induced after inoculation with Cucumber mosaic virus (CMV) or Rice necrosis mosaic virus (RNMV). After inoculating OsHAP2E::GUS-transgenic plants with either virus, the levels of GUS expression increased significantly. The expression levels of GUS or OsHAP2E reached a plateau 5 days after inoculation of rice with CMV, which paralleled the accumulation of CMV RNA level. Furthermore, transgenically over-expressed lines of OsHAP2E (OsHAP2E-OX) had lower levels of CMV and RNMV RNAs than in in nontransgenic control plants. The OsHAP2E-OX lines developed no significant symptoms from RNMV while control plants had yellowing and stunting. These results suggested that OsHAP2E is induced by virus infection and contributes to resistance against viral pathogens.

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Acknowledgments

We thank Drs. C. Masuta and T. Tachibana for the infested soil with P. graminis-carrying RNMV and the use of Retoratome, respectively. We are also grateful to D. Murphy for English editing of the manuscript. This work was supported by the Program for Promotion of Basic and Applied Researches in Bio-oriented Industry, and the Ministry of Education, Culture, Sports, Science and Technology of Japan [Grant-in-Aid for Scientific Research for Scientific Research (C) and (B), No. 24580065 to MN and No. 26292026 to KK] and by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project RMP-2113 to MN; Green Technology Project EF-1001 and EF-1004 to HI).

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  1. Hidemitsu Nakamura

    Present address: Department of Applied Biological Chemistry, Graduate School of Agricultural Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo, Bunkyo-ku, 113-8657, Japan

Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime, 790-8566, Japan

    Md. Mahfuz Alam, Kappei Kobayashi, Takashi Yaeno, Naoto Yamaoka & Masamichi Nishiguchi

  2. Faculty of Agriculture, Ehime University, Matsuyama, Ehime, 790-8566, Japan

    Kappei Kobayashi, Takashi Yaeno, Naoto Yamaoka & Masamichi Nishiguchi

  3. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Hidemitsu Nakamura & Hiroaki Ichikawa

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  1. Md. Mahfuz Alam
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Correspondence to Masamichi Nishiguchi.

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Alam, M.M., Nakamura, H., Ichikawa, H. et al. Overexpression of OsHAP2E for a CCAAT-binding factor confers resistance to Cucumber mosaic virus and Rice necrosis mosaic virus . J Gen Plant Pathol 81, 32–41 (2015). https://doi.org/10.1007/s10327-014-0564-9

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