Abstract
Samples of Cucurbita maxima plants with foliar yellowing and curling symptoms were collected in Komae City (Japan) in 2011 and shown by Southern blot hybridization to be associated with a begomovirus. Rolling circle amplification and PCR-mediated amplification with specific primers showed the presence of a begomovirus and an alphasatellite, but neither a DNA-B component or a betasatellite. Sequence analysis of full-length clones showed the virus to be an isolate of the Israel strain of Tomato yellow leaf curl virus and the alphasatellite Sida yellow vein China alphasatellite. This is the first identification of these components in a cucurbit in Japan.
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Acknowledgments
This research was supported in part by an Advanced Research Project of the Nodai Research Institute, Tokyo University of Agriculture, Japan. RWB was supported by the Higher Education Commission (Govt. of Pakistan) under the Foreign Faculty Hiring Program.
Ethical standards
All the experiments were performed in accordance with the current regulations for agriculture crops of Japan.
Conflict of interest
All the authors read and approved the final manuscript, and the authors have no conflict of interest to declare.
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Shahid, M.S., Ikegami, M., Briddon, R.W. et al. Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan. J Gen Plant Pathol 81, 92–95 (2015). https://doi.org/10.1007/s10327-014-0563-x
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DOI: https://doi.org/10.1007/s10327-014-0563-x