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First report of broccoli foot rot caused by Rhizoctonia solani AG-2-2 IV and pathogenicity comparison of the pathogen with related pathogens

Journal of General Plant Pathology volume 81, pages 15–23 (2015)Cite this article

Abstract

Foot rot and wilt symptoms were found on mature broccoli plants in Hokkaido, Japan, in 2007. The causal fungus was identified as Rhizoctonia solani anastomosis group (AG)-2-2 IV based on a hyphal fusion test, cultural characteristics and DNA analysis. We compared the isolate with two reference isolates, the pathogen of broccoli damping-off, belonging to AG-1 IC and AG-2-2 IIIB, in terms of differences in virulence to broccoli plants at different growth stages under various conditions. All the isolates caused damping-off on all three broccoli cultivars tested. However, only the present isolate incited foot rot with wilt symptoms on mature plants, when temperature was optimum for plant growth (20–25 °C), indicating that the foot rot pathogen may be discriminated from the damping-off pathogen in terms of virulence to mature broccoli. We also compared the isolate with 10 reference isolates from various crops, belonging to AG-2-2 IV, AG-2-2 IIIB and AG-1 IC, in terms of virulence to broccoli. All the isolates caused damping-off on broccoli seedlings with one exception; however, only the present isolate and one of four AG-2-2 IV isolates tested caused foot rot with wilt symptoms on mature broccoli in all the replicates. These results suggest that a group of isolates among AG-2-2 IV population is capable of causing foot rot on mature broccoli plants. This is the first report of broccoli foot rot caused by R. solani AG-2-2 IV.

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Acknowledgments

We thank Dr. N. Matsumoto (Hokkaido University, Japan) for valuable comments and English correction of the manuscript and Y. Kishida (Hokkaido Prefectural Hiyama Agricultural Extension Center, Japan) for sampling support.

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Authors and Affiliations

  1. Donan Agricultural Experiment Station, Hokkaido Research Organization, Hokuto, Hokkaido, 041-1201, Japan

    Tomoo Misawa

  2. National Agriculture and Food Research Organization, Institute of Vegetable and Tea Science, Tsukuba, Ibaraki, 305-8666, Japan

    Masaharu Kubota

  3. Kitami Agricultural Experiment Station, Hokkaido Research Organization, Kun-Neppu, Hokkaido, 099-1496, Japan

    Jun Sasaki

  4. Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Shiro Kuninaga

Authors
  1. Tomoo Misawa
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  2. Masaharu Kubota
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  3. Jun Sasaki
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  4. Shiro Kuninaga
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Corresponding author

Correspondence to Tomoo Misawa.

Additional information

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions AB911320–911323.

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Misawa, T., Kubota, M., Sasaki, J. et al. First report of broccoli foot rot caused by Rhizoctonia solani AG-2-2 IV and pathogenicity comparison of the pathogen with related pathogens. J Gen Plant Pathol 81, 15–23 (2015). https://doi.org/10.1007/s10327-014-0551-1

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  • DOI: https://doi.org/10.1007/s10327-014-0551-1

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