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A useful method for preparing microsclerotial inoculum of Verticillium dahliae

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Abstract

Various methods of inoculating plants with Verticillium dahliae have been described; however, so far, there are no effective preparations of its microsclerotia, a major source of infection under natural field conditions. In this study, we developed a simple and effective method to prepare microsclerotial inoculum of V. dahliae using a cellophane medium that effectively promoted microsclerotia formation. In addition, a sieving process allowed the preparation of inoculum exclusively composed of microsclerotia. Our novel method provides a reproducible and stable inoculum for disease induction by V. dahliae under conditions that approximate natural infections.

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Acknowledgments

We thank Drs. Hiroyuki Nieda, Nobuo Hayashi, Koji Hashimoto, and Tsutomu Iijima for useful advice and Drs. Kenro Ohshima, and Shigetou Namba for critical reading of the manuscript and valuable comments.

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Authors and Affiliations

  1. Gunma Agricultural Technology Center, Gunma, 493 Nishiobokata, Isesaki, Gunma, 379-2224, Japan

    Toshimasa Shiraishi & Kentaro Ikeda

  2. Gunma Prefectural Office, 1-1-1 Ote, Maebashi, Gunma, 371-8570, Japan

    Hiroshi Sakai & Toshihiko Urushibara

Authors
  1. Toshimasa Shiraishi
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  2. Hiroshi Sakai
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  3. Kentaro Ikeda
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  4. Toshihiko Urushibara
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Correspondence to Toshimasa Shiraishi.

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Shiraishi, T., Sakai, H., Ikeda, K. et al. A useful method for preparing microsclerotial inoculum of Verticillium dahliae . J Gen Plant Pathol 80, 475–478 (2014). https://doi.org/10.1007/s10327-014-0549-8

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  • DOI: https://doi.org/10.1007/s10327-014-0549-8

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