Pythium species were isolated from seedlings of strawberry with root and crown rot. The isolates were identified as P. helicoides on the basis of morphological characteristics and sequences of the ribosomal DNA internal transcribed spacer regions. In pathogenicity tests, the isolates caused root and crown rot similar to the original disease symptoms. Multiplex PCR was used to survey pathogen occurrence in strawberry production areas of Japan. Pythium helicoides was detected in 11 of 82 fields. The pathogen is distributed over six prefectures.
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We thank K. Suzuki (Chiba Prefectural Agriculture and Forestry Research Center, Japan), M. Inada (Saga Agricultural Research Center, Japan), K. Onodera (Miyoshi Co. Ltd., Japan), Y. Hirayama (Nara Prefectural Agricultural Experiment Station, Japan), S. Morishima (Tochigi Prefectural Agricultural Experiment Station, Japan), M. Sumino (Hokkaido Research Organization, Agriculture Research Department Ornamental Plants and Vegetables Research Center, Japan) and T. Funakubo (Yamanashi Prefectural Agritechnology Center, Japan) for providing soil samples from strawberry planting areas.
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Ishiguro, Y., Otsubo, K., Watanabe, H. et al. Root and crown rot of strawberry caused by Pythium helicoides and its distribution in strawberry production areas of Japan. J Gen Plant Pathol 80, 423–429 (2014). https://doi.org/10.1007/s10327-014-0520-8