Abstract
Pythium species were isolated from seedlings of strawberry with root and crown rot. The isolates were identified as P. helicoides on the basis of morphological characteristics and sequences of the ribosomal DNA internal transcribed spacer regions. In pathogenicity tests, the isolates caused root and crown rot similar to the original disease symptoms. Multiplex PCR was used to survey pathogen occurrence in strawberry production areas of Japan. Pythium helicoides was detected in 11 of 82 fields. The pathogen is distributed over six prefectures.
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References
Funakubo T, Kageyama K, Uematsu S (2009) Occurrence of root rot of Erica formasa caused by Pythium helicoides (Abstract in Japanese). Jpn J Phytopathol 75:186
Ishiguro Y, Asano T, Otsubo K, Suga H, Kageyama K (2013) Simultaneous detection by multiplex PCR of the high-temperature-growing Pythium species: P. aphanidermatum, P. helicoides and P. myriotylum. J Gen Plant Pathol 79:350–358
Ishiguro Y, Otsubo K, Watarai M, Iwashita T, Tomita M, Suematsu M, Fukui H, Suga H, Kageyama K (2014) Seedling blight of Glycyrrhiza uralensis caused by Pythium myriotylum, P. aphanidermatum and P. spinosum, and identifying primary inoculum sources using multiplex PCR detection. J Gen Plant Pathol (in press). doi:10.1007/s10327-014-0508-4
Kageyama K (2010) Mycobiota in the subtropical and cool temperate areas in Japan (in Japanese). IFO Res Commun 24:117–156
Kageyama K (2011) Characteristics of high-temperature-growing Pythium species nowadays frequently occurring disease (in Japanese). Plant Prot 65:102–106
Kageyama K, Aoyagi T, Sunouchi R, Fukui H (2002) Root rot of miniature roses caused by Pythium helicoides. J Gen Plant Pathol 68:15–20
Li M, Asano T, Suga H, Kageyama K (2011) A multiplex PCR for the detection of Phytophthora nicotianae and P. cactorum, and a survey of their occurrence in strawberry production areas of Japan. Plant Dis 95:1270–1278
Li M, Inada M, Watanabe H, Suga H, Kageyama K (2013) Simultaneous detection and quantification of Phytophthora nicotianae and P. cactorum, and distribution analyses in strawberry greenhouses by duplex real-time PCR. Microbes Environ 28:195–203
Matsuzaki M, Kan M, Kiso A (1980) Phytophthora rot of strawberry in Kyushu, Japan (in Japanese). Ann Phytopath Soc Japan 46:179–184
Matuo T (1972) Taxonomic studies on phytopathogenic fusaria (in Japanese). Ann Phytopath Soc Japan 38:167–169
Miyake N, Nagai H, Fukuta S, Kuroyanagi S, Takahashi R, Kageyama K (2012) Occurrence of Pythium root rot of poinsettia caused by Pythium myriotylum Drechsler and Pythium helicoides Drechsler (Abstract in Japanese). Jpn J Phytopathol 78:183
Miyazaki A, Watanabe H, Kageyama K (2009) First report of root rot on begonia (Begonia × hiemalis) caused by Pythium helicoides Drechsler in Japan (in Japanese). Ann Rept Kansai Pl Prot 51:53–54
Morita Y, Tojo M (2007) Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil. Plant Dis 91:1591–1599
Nakayama K, Kageyama K, Watanabe H, Fukuda T, Ishikawa S (2008) Occurrence of Pythium root rot of strawberry caused by Pythium spinosum and P. sylvaticum (Abstract in Japanese). Jpn J Phytopathol 74:177
Sugawara K, Kageyama K, Satou M, Namai T (2010) Pythium rot of alstroemeria caused by Pythium myriotylum and P. helicoides (Abstract in Japanese). Jpn J Phytopathol 76:44
Suzui T, Makino T, Ogoshi A (1980) Phytophthora rot of strawberry caused by Phytophthora nicotianae var. parasitica in Shizuoka. Ann Phytopath Soc Japan 46:169–178
Suzuki M, Togawa M, Yoneyama C (2005) Occurrence of Pythium root rot of strawberry caused by Pythium helicoides (Abstract in Japanese). Jpn J Phytopathol 71:209
Suzuki M, Kageyama K, Ichikawa T, Uchiyama T (2009) Occurrence of Pythium root rot of gerbera caused by Pythium helicoides (Abstract in Japanese). Jpn J Phytopathol 75:237
Tojo M, Fujita Y, Awad HM, Ichitani T (1993) Preparation of Pythium inocula using bentgrass seeds for glasshouse studies (in Japanese). Proc Kansai Pl Prot 35:1–5
van der Plaats-Niterink AJ (1981) Monograph of the genus Pythium. Stud Mycol 21:1–242
Watanabe T (1977) Pathogenicity of Pythium myriotylum isolated from strawberry roots in Japan. Ann Phytopath Soc Japan 43:306–309
Watanabe T, Hashimoto K, Sato M (1977) Pythium species associated with strawberry roots in Japan, and their role in the strawberry stunt disease. Phytopathology 67:1324–1332
Watanabe H, Horinouchi H, Tanahashi I, Kageyama K (2005) Occurrence of root rot of strawberry caused by Pythium helicoides, and pathogenicity to several crops (Abstract in Japanese). Jpn J Phytopathol 71:209–210
Watanabe H, Taguchi Y, Hyakumachi M, Kageyama K (2007) Pythium and Phytophthora species associated with root and stem rots of kalanchoe. J Gen Plant Pathol 73:81–88
Waterhouse GM (1967) Key to Pythium Pringsheim. Commonw Mycol Inst Kew UK Mycol Pap 109:1–15
Acknowledgments
We thank K. Suzuki (Chiba Prefectural Agriculture and Forestry Research Center, Japan), M. Inada (Saga Agricultural Research Center, Japan), K. Onodera (Miyoshi Co. Ltd., Japan), Y. Hirayama (Nara Prefectural Agricultural Experiment Station, Japan), S. Morishima (Tochigi Prefectural Agricultural Experiment Station, Japan), M. Sumino (Hokkaido Research Organization, Agriculture Research Department Ornamental Plants and Vegetables Research Center, Japan) and T. Funakubo (Yamanashi Prefectural Agritechnology Center, Japan) for providing soil samples from strawberry planting areas.
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Ishiguro, Y., Otsubo, K., Watanabe, H. et al. Root and crown rot of strawberry caused by Pythium helicoides and its distribution in strawberry production areas of Japan. J Gen Plant Pathol 80, 423–429 (2014). https://doi.org/10.1007/s10327-014-0520-8
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DOI: https://doi.org/10.1007/s10327-014-0520-8