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Progeny derived from Thanatephorus cucumeris (Rhizoctonia solani) AG-2-2 IV isolates on sugar beet foliage show more clonal diversity in somatic compatibility grouping than those from roots and petioles

  • Fungal Diseases
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Abstract

Twenty-nine isolates of Thanatephorus cucumeris (Rhizoctonia solani) AG-2-2 IV were collected from the roots, petioles, and leaves of diseased sugar beets in Hokkaido, Japan. We examined the genetic variation of the field isolates using somatic compatibility grouping of progeny (cultured isolates derived from induced basidiospores) based on analysis of hyphal anastomosis reactions (i.e., hyphal perfect fusion) and inter-simple sequence repeats (ISSRs). The number of somatic compatibility groups (SCGs) of single basidiospore isolates was strongly correlated with the host plant tissue from which the parental isolates were obtained. Parental isolates from roots and petioles tended to be genetically heterogeneous and generated plural SCGs, whereas isolates (except for two non-self-anastomosing isolates) from leaves tended to be homogeneous and generated a single SCG. Heterogeneous sibling basidiospore isolates yielded homogeneous progeny within a few generations. These findings were supported by the results of ISSR analysis, which showed that the homogeneous isolates generated progeny with the same genotype, whereas heterogeneous isolates generated progeny with different genotypes. However, like SCGs, ISSR genotypes of heterogeneous progeny tended to be homogeneous within two or three generations. Additionally, we examined clonal diversity during the basidiospore infection process over a 2-year period. A heterogeneous isolate generated a large number of progeny SCGs, thereby increasing clonal diversity. In contrast, progeny SCGs were almost all the same in a homogeneous isolate plot with a few exceptions. These results indicate that infection of T. cucumeris basidiospores play a role in the clonal diversity of AG-2-2 IV isolates, as indicated by progeny SCGs in the field.

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Acknowledgments

This work was done in part at Hokkaido National Agricultural Experiment Station in Sapporo and in part at Hokkaido University. The authors thank Dr. Tsukiboshi of the National Institute of Livestock and Grasslands Science, Japan for invaluable comments and useful suggestions and Mr. M. J. Christensen for critically reading the manuscript.

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Author notes

  1. Takako Kiyoshi

    Present address: NARO Institute of Livestock and Grassland Science, Senbonmatsu 768, Nasushiobara, Tochigi, 329-2793, Japan

  2. Shigeo Naito

    Present address: Japan Association for Techno-innovation in Agriculture, Forestry and Fisheries, Sankaido Bldg., Akasaka 1-9-13, Tokyo, 107-0052, Japan

  3. Seishi Akino & Norio Kondo

    Present address: Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9 Kita-ku, Sapporo, 060-8589, Japan

  4. Sunao Ochi

    Present address: NARO Agricultural Research Center, Kannondai 3-1-1, Tsukuba, Ibaraki, 305-8666, Japan

Authors and Affiliations

  1. Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9 Kita-ku, Sapporo, 060-8589, Japan

    Takako Kiyoshi, Shigeo Naito, Seishi Akino, Sunao Ochi & Norio Kondo

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  1. Takako Kiyoshi
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Correspondence to Takako Kiyoshi.

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Kiyoshi, T., Naito, S., Akino, S. et al. Progeny derived from Thanatephorus cucumeris (Rhizoctonia solani) AG-2-2 IV isolates on sugar beet foliage show more clonal diversity in somatic compatibility grouping than those from roots and petioles. J Gen Plant Pathol 80, 136–146 (2014). https://doi.org/10.1007/s10327-013-0499-6

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