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Phenotypic characterization of colony morphological mutants of Burkholderia glumae that emerged during subculture

  • Bacterial and Phytoplasma Diseases
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Abstract

Burkholderia glumae causes rice seedling rot and grain rot disease. It is genetically unstable in its pathogenicity, and colony morphological mutants (CMMs) also frequently emerge during subculture. In this study, three types of CMM were isolated during subculture, and we characterized virulence factors such as phytotoxin, oxalic acid, and N-acylhomoserine lactone (AHL) productivity and motility. The CMMs had various phenotypes and loss of pathogenicity or reduction of virulence on seedlings and spikelets. Quorum sensing system (QSS) plays a central role in the virulence of B. glumae. Although the phytotoxin production and motility were lost or reduced in CMMs, AHLs were produced normally by 16 h after incubation, and no mutations were associated with genes for QSS such as tofI, tofR and qsmR. Thus, QSS in the CMMs seemed to function normally, with virulence reduced by another unknown mechanism. Furthermore, culture pH values decreased in the wild-type strain but increased in the CMMs at 30 °C in Luria–Bertani medium. Since many bacteria have been known to increase pH values in LB medium, culture acidification in LB medium may be a specific character to B. glumae, and reduction or loss of phytotoxin production, motility and pathogenicity of the CMMs might be affected by the reduction in acid productivity. Studies on naturally emerged CMMs may enable us to understand any novel adaptive mechanism specific to B. glumae.

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Authors and Affiliations

  1. Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, 321-8585, Japan

    Taro Kato, Tomohiro Morohoshi & Tsukasa Ikeda

  2. National Institute of Agro-Environment Sciences, Natural Resource Inventory Center, Tsukuba, Japan

    Seiya Tsushima

Authors
  1. Taro Kato
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  2. Tomohiro Morohoshi
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  3. Seiya Tsushima
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  4. Tsukasa Ikeda
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Correspondence to Tsukasa Ikeda.

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Kato, T., Morohoshi, T., Tsushima, S. et al. Phenotypic characterization of colony morphological mutants of Burkholderia glumae that emerged during subculture. J Gen Plant Pathol 79, 249–259 (2013). https://doi.org/10.1007/s10327-013-0454-6

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  • DOI: https://doi.org/10.1007/s10327-013-0454-6

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