Journal of General Plant Pathology

, Volume 79, Issue 1, pp 56–63 | Cite as

Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome

  • Kappei Kobayashi
  • Go Atsumi
  • Yasuya Iwadate
  • Reiko Tomita
  • Ken-ichi Chiba
  • Shiho Akasaka
  • Masahiro Nishihara
  • Hideyuki Takahashi
  • Naoto Yamaoka
  • Masamichi Nishiguchi
  • Ken-Taro Sekine
Viral and Viroid Diseases

Abstract

A virus-like dsRNA of about 23 kbp was detected in gentian plants showing Kobu-sho syndrome including stunting, shortened internodes, and tumors on stems, nodes and roots. Nucleotide sequence analysis has suggested that this dsRNA is related to Pestivirus species but not to any other plant viruses. It was protected from externally added RNase, suggesting that the dsRNA is encapsidated. The dsRNA was co-extracted in a crude homogenate of glutaraldehyde-fixed tissue with the virus-like particles that have been associated previously with Kobu-sho syndrome in gentian (Usugi et al. Jpn J Phytopathol 76:21–24, 2010). The RNA sequence was detected in more than 99 % of Kobu-sho gentian individuals but in less than 20 % of apparently healthy gentian individuals from fields affected with Kobu-sho. Thus, we propose naming the virus Gentian Kobu-sho-associated virus.

Keywords

Gentian Kobu-sho dsRNA Virus 

Supplementary material

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Supplementary material 1 (XLS 37 kb)
10327_2012_423_MOESM2_ESM.ppt (226 kb)
Supplementary material 2 (PPT 226 kb)
10327_2012_423_MOESM3_ESM.txt (4 kb)
Supplementary material 3 (TXT 5 kb)
10327_2012_423_MOESM4_ESM.txt (6 kb)
Supplementary material 4 (TXT 6 kb)

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Copyright information

© The Phytopathological Society of Japan and Springer Japan 2012

Authors and Affiliations

  • Kappei Kobayashi
    • 1
    • 2
  • Go Atsumi
    • 1
  • Yasuya Iwadate
    • 3
  • Reiko Tomita
    • 1
  • Ken-ichi Chiba
    • 3
  • Shiho Akasaka
    • 3
  • Masahiro Nishihara
    • 1
  • Hideyuki Takahashi
    • 1
  • Naoto Yamaoka
    • 2
  • Masamichi Nishiguchi
    • 2
  • Ken-Taro Sekine
    • 1
  1. 1.Iwate Biotechnology Research CenterKitakamiJapan
  2. 2.Faculty of AgricultureEhime UniversityMatsuyamaJapan
  3. 3.Iwate Agricultural Research CenterKitakamiJapan

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